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Biological Activity for Flutax 2
Flutax 2 is a green fluorescent taxol derivative. Binds microtubules with high affinity (Ka ~ 107M-1). Can be used for imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions. Binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive, and is more photostable than Flutax 1 (Cat. No. 2226). Excitation/emission maximum λ ~ 496/526 nm.
Flutax 2-labeled HeLa Cells for Flutax 2
Application of Flutax 2: Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.
Technical Data for Flutax 2
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Flutax 2
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Flutax 2
The following data is based on the product molecular weight 1319.28. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.01 mM||75.8 mL||378.99 mL||757.99 mL|
|0.05 mM||15.16 mL||75.8 mL||151.6 mL|
|0.1 mM||7.58 mL||37.9 mL||75.8 mL|
|0.5 mM||1.52 mL||7.58 mL||15.16 mL|
References for Flutax 2
References are publications that support the biological activity of the product.
Lillo et al (2002) Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids. Biochemistry. 41 12436 PMID: 12369834
Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101
If you know of a relevant reference for Flutax 2, please let us know.
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Citations for Flutax 2
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