Flutax 2

Pricing Availability   Qty
Description: Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions
Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for Flutax 2

Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.

Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.

Application: fluorescence microscopy.

Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.

Scientific Data

Fluorescence Imaging Application of Flutax 2 for labeling HeLa cells View Larger

Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.

Optical Data for Flutax 2

Flutax 2 Dye Spectra
λabs 496 nm
λem 526 nm
Extinction Coefficient (ε) 80000 M-1cm-1
Quantum Yield (φ) 0.9
Application Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Flutax 2

M. Wt 1319.28
Formula C71H64F2N2O21
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 301844-13-3
PubChem ID 44571006
Smiles O=C1[C@@]2(C)C([C@@]3(OC(C)=O)[C@H](OC3)C[C@@H]2OC([C@H](C)NC(C4=CC=C(C5=C(C=C6F)C(OC7=C5C=C(F)C(O)=C7)=CC6=O)C(C(O)=O)=C4)=O)=O)[C@H](OC(C8=CC=CC=C8)=O)[C@@]9(O)C(C)(C)C([C@H]1OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H](C%10=CC=CC=C%10)NC(C%11=CC=CC=C%11)=O)=O)C9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Flutax 2

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 1.32 1

Preparing Stock Solutions for Flutax 2

The following data is based on the product molecular weight 1319.28. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 75.8 mL 378.99 mL 757.99 mL
0.05 mM 15.16 mL 75.8 mL 151.6 mL
0.1 mM 7.58 mL 37.9 mL 75.8 mL
0.5 mM 1.52 mL 7.58 mL 15.16 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Flutax 2

Certificate of Analysis / Product Datasheet
Select another batch:

References for Flutax 2

References are publications that support the biological activity of the product.

Lillo et al (2002) Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids. Biochemistry. 41 12436 PMID: 12369834

Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101

If you know of a relevant reference for Flutax 2, please let us know.

Keywords: Flutax 2, Flutax 2 supplier, paclitaxel, Flutax2, Taxol, fluorescent, dye, labeled, labelled, microtubules, tubulin, oregon, green, Microtubules, Mitosis, Organelle, and, Cell, Structure, Stains, Microtubule, Probes, 6254, Tocris Bioscience

Citations for Flutax 2

Citations are publications that use Tocris products.

Currently there are no citations for Flutax 2. Do you know of a great paper that uses Flutax 2 from Tocris? Please let us know.

Reviews for Flutax 2

There are currently no reviews for this product. Be the first to review Flutax 2 and earn rewards!

Have you used Flutax 2?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals