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Biological Activity for Flutax 1
Flutax 1 is a a fluorescent taxol derivative that binds to the taxol microtubule binding site with high affinity (Ka ~ 107M-1). Useful for direct imaging of the microtubule cytoskeleton. Excitation maximum ~ 495 nm; emission maximum ~ 520 nm.
Flutax 1-labeled HeLa Cells
Application of Flutax 1: Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal.
Technical Data for Flutax 1
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Flutax 1
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Flutax 1
The following data is based on the product molecular weight 1283.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||0.78 mL||3.9 mL||7.79 mL|
|5 mM||0.16 mL||0.78 mL||1.56 mL|
|10 mM||0.08 mL||0.39 mL||0.78 mL|
|50 mM||0.02 mL||0.08 mL||0.16 mL|
References for Flutax 1
References are publications that support the biological activity of the product.
Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101
Diaz et al (2003) Fast kinetics of Tax. binding to microtubules. J.Biol.Chem. 278 8407 PMID: 12496245
Evangelio et al (1998) Fluorescent taxoids as probes of the microtubule cytoskeleton. Cell Motil.Cytoskel. 39 73
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2 Citations for Flutax 1
Citations are publications that use Tocris products. Selected citations for Flutax 1 include:
Kaittanis et al (2015) Multifunctional MRI/PET Nanobeacons Derived from the in Situ Self-Assembly of Translational Polymers and Clinical Cargo through Coalescent Intermolecular Forces. Nano Lett 15 8032 PMID: 26540670
Kaittanis et al (2014) Environment-responsive nanophores for therapy and treatment monitoring via molecular MRI quenching. Nat Commun 5 3384 PMID: 24594970
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Literature in this Area
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