You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Polyene antibiotic. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form. Also fluorescent cholesterol stain.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 654.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.05 mM||30.54 mL||152.71 mL||305.42 mL|
|0.25 mM||6.11 mL||30.54 mL||61.08 mL|
|0.5 mM||3.05 mL||15.27 mL||30.54 mL|
|2.5 mM||0.61 mL||3.05 mL||6.11 mL|
References are publications that support the biological activity of the product.
Castanho et al (1992) Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J.Biol.Chem. 267 204 PMID: 1730589
Marella et al (2002) Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. J.Biol.Chem. 277 25457 PMID: 11994310
Pipalia et al (2006) Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells. J.Lipid Res. 47 284 PMID: 16288097
If you know of a relevant reference for Filipin III, please let us know.
View Related Products by Target
Keywords: Filipin III, Filipin III supplier, polyene, antibiotics, antifungals, prions, protein, inhibitors, inhibits, PrP, fluorescent, cholesterol, stain, Antibiotics, Prion, Protein, Labels, and, Stains, Fluorescent, Cholesterol, Probes, Lipid, Cell, Membrane, Antifungals, 6250, Tocris Bioscience
Citations for Filipin III
Citations are publications that use Tocris products.
Currently there are no citations for Filipin III. Do you know of a great paper that uses Filipin III from Tocris? Please let us know.
Reviews for Filipin III
Average Rating: 5 (Based on 1 Review.)
Have you used Filipin III?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Fix the cells with 4% PFA for 15 min at room temperature; permeabilisation not required; incubate the cells with 0.5 mg/mL Fillipin in 3% BSA for 4 h at room temperature; mount the coverslips with DAPI-free moviol. Excite the cells with 405 nm laser, and collect signals at 410-500 nm.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide [Spring/Summer 2019]
Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells