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Polyene antibiotic. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form. Also fluorescent cholesterol stain.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 654.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.05 mM||30.54 mL||152.71 mL||305.42 mL|
|0.25 mM||6.11 mL||30.54 mL||61.08 mL|
|0.5 mM||3.05 mL||15.27 mL||30.54 mL|
|2.5 mM||0.61 mL||3.05 mL||6.11 mL|
References are publications that support the biological activity of the product.
Castanho et al (1992) Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J.Biol.Chem. 267 204 PMID: 1730589
Marella et al (2002) Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. J.Biol.Chem. 277 25457 PMID: 11994310
Pipalia et al (2006) Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells. J.Lipid Res. 47 284 PMID: 16288097
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Keywords: Filipin III, Filipin III supplier, polyene, antibiotics, antifungals, prions, protein, inhibitors, inhibits, PrP, fluorescent, cholesterol, stain, Antibiotics, Prion, Protein, Labels, and, Stains, 6250, Tocris Bioscience
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Fix the cells with 4% PFA for 15 min at room temperature; permeabilisation not required; incubate the cells with 0.5 mg/mL Fillipin in 3% BSA for 4 h at room temperature; mount the coverslips with DAPI-free moviol. Excite the cells with 405 nm laser, and collect signals at 410-500 nm.
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