Reagent for maintaining SH groups in the reduced state.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 154.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||6.48 mL||32.41 mL||64.83 mL|
|5 mM||1.3 mL||6.48 mL||12.97 mL|
|10 mM||0.65 mL||3.24 mL||6.48 mL|
|50 mM||0.13 mL||0.65 mL||1.3 mL|
References are publications that support the products' biological activity.
Rüegg and Rudinger (1977) Reductive cleavage of cystine disulfides with tributylphosphine. Methods Enzymol. 47 111 PMID: 927167
Sears et al (1977) Relative susceptibilities of the interchain disulfides of an immunoglobulin G molecule to reduction by dithiothreitol. Biochemistry 16 2031 PMID: 403938
Jabusch et al (1978) The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins. Anal.Biochem. 91 532 PMID: 9762140
Cleland (1964) Dithiothreitol, a new protective group for SH groups. Biochemistry 3 480 PMID: 14192894
If you know of a relevant reference for DL-Dithiothreitol, please let us know.
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