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DL-Dithiothreitol is a reagent for maintaining SH groups in the reduced state.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 154.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||6.48 mL||32.41 mL||64.83 mL|
|5 mM||1.3 mL||6.48 mL||12.97 mL|
|10 mM||0.65 mL||3.24 mL||6.48 mL|
|50 mM||0.13 mL||0.65 mL||1.3 mL|
References are publications that support the biological activity of the product.
Rüegg and Rudinger (1977) Reductive cleavage of cystine disulfides with tributylphosphine. Methods Enzymol. 47 111 PMID: 927167
Sears et al (1977) Relative susceptibilities of the interchain disulfides of an immunoglobulin G molecule to reduction by dithiothreitol. Biochemistry 16 2031 PMID: 403938
Jabusch et al (1978) The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins. Anal.Biochem. 91 532 PMID: 9762140
Cleland (1964) Dithiothreitol, a new protective group for SH groups. Biochemistry 3 480 PMID: 14192894
If you know of a relevant reference for DL-Dithiothreitol, please let us know.
Keywords: DL-Dithiothreitol, DL-Dithiothreitol supplier, Maintains, SH, groups, reduced, state, Reagents, Clelands, reagent, DTT, Cleland's, Sputolysin, Biochemicals, and, Molecular, Biology, 3154, Tocris Bioscience
Citations are publications that use Tocris products. Selected citations for DL-Dithiothreitol include:
Baca et al (2013) Gene-environment interactions affect long-term depression (LTD) through changes in DA receptor affinity in Snap25 deficient mice. Brain Res 1532 85 PMID: 23939223
Do you know of a great paper that uses DL-Dithiothreitol from Tocris? Please let us know.
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