DL-Dithiothreitol

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Cat.No. 3154 - DL-Dithiothreitol | C4H10O2S2 | CAS No. 3483-12-3
Description: Maintains SH groups in the reduced state
Alternative Names: DTT, Cleland's reagent, Sputolysin
Chemical Name: threo-1,4-Dimercapto-2,3-butanediol
Datasheet
Citations
Literature

Biological Activity

Reagent for maintaining SH groups in the reduced state.

Technical Data

M. Wt 154.25
Formula C4H10O2S2
Storage Store at +4°C
CAS Number 3483-12-3
PubChem ID 51341375
InChI Key DCRJBUYBNCQKEE-CKQOXSRLSA-N
Smiles SC[C@H](O)[C@@H](O)CS.SC[C@@H](O)[C@H](O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 15.43 100
water 15.42 100

Preparing Stock Solutions

The following data is based on the product molecular weight 154.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.48 mL 32.41 mL 64.83 mL
5 mM 1.3 mL 6.48 mL 12.97 mL
10 mM 0.65 mL 3.24 mL 6.48 mL
50 mM 0.13 mL 0.65 mL 1.3 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Rüegg and Rudinger (1977) Reductive cleavage of cystine disulfides with tributylphosphine. Methods Enzymol. 47 111 PMID: 927167

Sears et al (1977) Relative susceptibilities of the interchain disulfides of an immunoglobulin G molecule to reduction by dithiothreitol. Biochemistry 16 2031 PMID: 403938

Jabusch et al (1978) The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins. Anal.Biochem. 91 532 PMID: 9762140

Cleland (1964) Dithiothreitol, a new protective group for SH groups. Biochemistry 3 480 PMID: 14192894


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Keywords: Maintains SH groups reduced state Reagents Clelands reagent DTT Cleland's reagent Sputolysin Biochemicals and Molecular Biology

Citations for DL-Dithiothreitol

Citations are publications that use Tocris products.

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Literature in this Area

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Pathways for DL-Dithiothreitol

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