Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewASP 7967 New
Description: Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches
Chemical Name: N2,N4-Bis(4-fluorophenyl)-N6-(2-pyrimidinylmethyl)-1,3,5-triazine-2,4,6-triamine hemifumarate
Purity: ≥98% (HPLC)
Biological Activity for ASP 7967
ASP 7967 is a ligand for RNA aptamer AC17-4 that activates gene expression in synthetic aptazime-based riboswitches at as low as 5 μM concentration, without relying on exogenous protein factors. ASP 7967 binds aptamer AC17-4 with high affinity in HEK293 cells (Kd = 12 nM; EC50 = 2.3 μM), activating gene expression (>10-fold) in HEK293 cells. ON/OFF ratio of riboswitches activated by ASP 7967 approaches 300, with a low basal expression level in cultured cells.
In vivo, ASP 7967 upregulates the serum hEPO concentration by 7.2-fold over the vehicle control (mice). Cell permeable and low-toxic, ASP 7967 is orally bioavailable.
Technical Data for ASP 7967
| M. Wt | 464.44 |
| Formula | C20H16F2N8.½C4H4O4 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1246958-42-8 |
| PubChem ID | 46931459 |
| InChI Key | PQVNSJGSJVAEAC-WXXKFALUSA-N |
| Smiles | FC1=CC=C(C=C1)NC2=NC(NCC3=NC=CC=N3)=NC(NC4=CC=C(F)C=C4)=N2.OC(/C=C/C(O)=O)=O.FC5=CC=C(C=C5)NC6=NC(NCC7=NC=CC=N7)=NC(NC8=CC=C(F)C=C8)=N6 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ASP 7967
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 46.44 | 100 |
Preparing Stock Solutions for ASP 7967
The following data is based on the product molecular weight 464.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.15 mL | 10.77 mL | 21.53 mL |
| 5 mM | 0.43 mL | 2.15 mL | 4.31 mL |
| 10 mM | 0.22 mL | 1.08 mL | 2.15 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.43 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for ASP 7967
References for ASP 7967
References are publications that support the biological activity of the product.
Salado et al (2018) Optimization of the pharmacokinetic properties of potent anti trypanosomal triazine derivatives. Eur.J.Med.Chem. 151 18 PMID: 29604541
Teixeira et al (2024) Synthetic gene circuits for regulation of next-generation cell-based therapeutics. Adv.Sci. 11 PMID: 38126677
Fukunaga et al (2023) Small-molecule aptamer for regulating RNA functions in mammalian cells and animals. J.Am.Chem.Soc. 145 7820 PMID: 36991533
Takahashi et al (2024) RNA-based controllers for engineering gene and cell therapies. Curr.Opin.Biotech. 85 PMID: 38052131
Olenginski et al (2024) Flipping the script: understanding riboswitches from an alternative perspective. J.Biol.Chem. 300 PMID: 38336293
Kläge et al (2024) A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture. RNA Biology 21 1 PMID: 38146121
Wang et al (2023) Integrating ligands into nucleic acid systems. ChemBioChem 24 PMID: 37401635
If you know of a relevant reference for ASP 7967, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: ASP 7967, ASP 7967 supplier, ASP7967, specific, ligand, binds, RNA, aptamer, AC17-4, activates, activator, gene, expression, riboswitch, riboswitches, signal, transduction, cell, permeable, non-toxic, upregulates, hEPO, in, vivo, orally, bioavailable, DNA,, and, Protein, Synthesis, 8803, Tocris Bioscience
Citations for ASP 7967
Citations are publications that use Tocris products.
Currently there are no citations for ASP 7967. Do you know of a great paper that uses ASP 7967 from Tocris? Please let us know.
Reviews for ASP 7967
There are currently no reviews for this product. Be the first to review ASP 7967 and earn rewards!
Have you used ASP 7967?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product Guide
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Excitotoxicity
- Stem Cells