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Inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 342.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||14.6 mL||73.01 mL||146.02 mL|
|1 mM||2.92 mL||14.6 mL||29.2 mL|
|2 mM||1.46 mL||7.3 mL||14.6 mL|
|10 mM||0.29 mL||1.46 mL||2.92 mL|
References are publications that support the biological activity of the product.
Kim et al (2008) Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 4 659 PMID: 18424912
Sato et al (2007) Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 67 9677 PMID: 17942897
Tanada et al (2005) Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1 84 PMID: 16874052
Wilcox and Mason (1992) Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 285 495 PMID: 1637341
Hoffmann et al (2020) SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor. Cell 181 271 PMID: 32142651
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Keywords: E 64d, E 64d supplier, lysosomal, proteases, inhibitors, inhibits, cathepsins, autophagy, autolysosomal, digestion, E64d, COVID-19, SARS-Cov-2, Autophagy, Calcium-Sensitive, Proteases, Cathepsin, Coronavirus, 4545, Tocris Bioscience
2 Citations for E 64d
Citations are publications that use Tocris products. Selected citations for E 64d include:
Zhang et al (2017) TRPC5-induced autophagy promotes drug resistance in breast carcinoma via CaMKKβ/AMPKα/mTOR pathway. Sci Rep 7 3158 PMID: 28600513
Guerroue et al (2017) Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Mol Cell 68 786 PMID: 29149599
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