7-Aminoactinomycin D

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Cat.No. 7121 - 7-Aminoactinomycin D | C62H87N13O16 | CAS No. 7240-37-1
Description: Fluorescent DNA stain
Chemical Name: 2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Purity: ≥97% (HPLC)
Literature (1)

Biological Activity

Fluorescent DNA stain. G-C base-specific DNA intercalator that forms stable complexes with DNA. Suitable for use in flow cytometry. Detects apoptotic cells. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Excitation/emission maxima (λ) = 488/655 nm.

Technical Data

M. Wt 1270.45
Formula C62H87N13O16
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 7240-37-1
PubChem ID 14924508
Smiles NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 2 2

Preparing Stock Solutions

The following data is based on the product molecular weight 1270.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 39.36 mL 196.78 mL 393.56 mL
0.1 mM 7.87 mL 39.36 mL 78.71 mL
0.2 mM 3.94 mL 19.68 mL 39.36 mL
1 mM 0.79 mL 3.94 mL 7.87 mL

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References are publications that support the biological activity of the product.

Zelenin et al (1984) 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry 5 348 PMID: 6468175

Schmid et al (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13 204 PMID: 1547670

Philpott et al (1996) The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques. Blood 87 2244 PMID: 8630384

If you know of a relevant reference for 7-Aminoactinomycin D, please let us know.

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