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Submit Review7-Aminoactinomycin D
Description: Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy
Chemical Name: 2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Purity: ≥97% (HPLC)
Biological Activity for 7-Aminoactinomycin D
Key information: 7-aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain. It is membrane impermeant to live cells but penetrates dead or damaged cells.
Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.
Application: fluorescence microscopy and flow cytometry.
Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.
Optical Data for 7-Aminoactinomycin D
| λabs | 549 nm |
|---|---|
| λem | 655 nm |
| Application | Fluorescence Microscopy, Flow Cytometry |
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Technical Data for 7-Aminoactinomycin D
| M. Wt | 1270.45 |
| Formula | C62H87N13O16 |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 7240-37-1 |
| PubChem ID | 14924508 |
| InChI Key | YXHLJMWYDTXDHS-UHFFFAOYSA-N |
| Smiles | NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 7-Aminoactinomycin D
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2 | 2 |
Preparing Stock Solutions for 7-Aminoactinomycin D
The following data is based on the product molecular weight 1270.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.02 mM | 39.36 mL | 196.78 mL | 393.56 mL |
| 0.1 mM | 7.87 mL | 39.36 mL | 78.71 mL |
| 0.2 mM | 3.94 mL | 19.68 mL | 39.36 mL |
| 1 mM | 0.79 mL | 3.94 mL | 7.87 mL |
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Product Datasheets for 7-Aminoactinomycin D
References for 7-Aminoactinomycin D
References are publications that support the biological activity of the product.
Zelenin et al (1984) 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry 5 348 PMID: 6468175
Schmid et al (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13 204 PMID: 1547670
Philpott et al (1996) The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques. Blood 87 2244 PMID: 8630384
If you know of a relevant reference for 7-Aminoactinomycin D, please let us know.
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Citations for 7-Aminoactinomycin D
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