- Cell Biology
- Product Type
- Research Area
- New Products
- About Tocris
- Contact Us
This is intended as a guide only.
CEPT Cocktail is a combination of small molecules, Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), and trans-ISRIB (Cat. No. 5284) available from Tocris, which improves the viability of pluripotent stem cells (PSCs). This combination has been shown to be effective in cryopreservation, routine long-term passaging, as well as embryoid body and organoid formation.
|Chroman 1||7163||1 vial||10,000x||500 μL DMSO for a 0.5 mM
|Emricasan||7310||1 vial||10,000x||500 μL DMSO for a 50 mM
|trans-ISRIB||5284||1 vial||10,000x||500 μL DMSO for a 7 mM
|7739||1 vial||1000x||5 mL ddH2O|
|DMSO, sterile filtered||3176||5 mL|
1. Prepare stock solutions (10,000x) for Chroman 1 (0.5 mM), Emricasan (50 mM) and trans-ISRIB (7 mM) by adding 500 μL of DMSO, sterile filtered, to each vial. Mix thoroughly or gently warm the solutions in a 45-60ºC water bath to ensure complete reconstitution. Add 100 μL of resuspended 10,000x stock solution per 1 L of media.
2. Prepare a 1000x Polyamine Supplement stock solution by reconstituting vial contents in 5 mL of ddH2O and filter sterilize before use. Add 1 mL of sterile resuspended polyamines supplement (1000x stock) per 1 L of media.
3. Filter the prepared medium before use.
Store the stock solutions, once prepared, at -80°C and use them within 6 months, or at -20°C within 1 month.