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DMSO, sterile filtered
DMSO, sterile filtered is a solvent with wide ranging applications in cell culture, cryopreservation and organic chemistry. Dissolves both polar and nonpolar compounds. Improves responsiveness of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to differentiation signals. Used in PCR to inhibit secondary structure formation by DNA template or primers.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Chetty et al (2013) A simple tool to improve pluripotent stem cell differentiation. Nat.Methods 10 553 PMID: 23584186
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Keywords: DMSO, sterile filtered, DMSO, sterile filtered supplier, Solvent, Reagents, Methyl, sulfoxide, Biochemicals, and, Molecular, Biology, ESCs, iPSC, Buffers,, Solvents, Solutions, 3176, Tocris Bioscience
16 Citations for DMSO, sterile filtered
Citations are publications that use Tocris products. Selected citations for DMSO, sterile filtered include:
Olkhanud et al (2011) Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4+ T cells to T-regulatory cells. Cancer Res 71 3505 PMID: 21444674
Romo-Parra et al (2008) GABA actions in hippocampal area CA3 during postnatal development: differential shift from depolarizing to hyperpolarizing in somatic and dendritic compartments. Neuroscience 99 1523 PMID: 18216229
Hermanson et al (2012) Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation. J Neuroendocrinol 7 e43580 PMID: 22952710
Ho et al (2012) Photoperiodic changes in endocannabinoid levels and energetic responses to altered signalling at CB1 receptors in Siberian hamsters. Behav Brain Res 24 1030 PMID: 22420341
Lively et al (2018) Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels. Front Cell Neurosci 12 115 PMID: 29780305
Morizane and Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc 12 195 PMID: 28005067
Wagner et al (2013) The dorsomedial hypothalamus mediates stress-induced hyperalgesia and is the source of the pronociceptive peptide cholecystokinin in the rostral ventromedial medulla. Am J Physiol Lung Cell Mol Physiol 238 29 PMID: 23415792
Hughes et al (2013) Endogenous analgesic action of the pontospinal noradrenergic system spatially restricts and temporally delays the progression of neuropathic pain following tibial nerve injury. Mol Brain 154 1680 PMID: 23707289
Zechel et al (2016) Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons. Elife 5 PMID: 27935475
Gofman et al (2016) Relative Role of Akt, ERK and CREB in Alcohol-Induced Microglia P2X4R Receptor Expression. Alcohol Alcohol 51 647 PMID: 26946194
Ito et al (2015) Influenza induces IL-8 and GM-CSF secretion by human alveolar epithelial cells through HGF/c-Met and TGF-α/EGFR signaling. Mol Brain 308 L1178 PMID: 26033355
Obis et al (2015) The novel protein kinase C ε isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release. PLoS One 8 8 PMID: 25761522
Obis et al (2015) The novel protein kinase C ε isoform modulates acetylcholine release in the rat neuromuscular junction. J Neurophysiol 8 80 PMID: 26625935
Ji et al (2014) L-type Ca2+ channel responses to bay k 8644 in stem cell-derived cardiomyocytes are unusually dependent on holding potential and charge carrier. Assay Drug Dev Technol 12 352 PMID: 25147907
Chen (2014) The development of zebrafish tendon and ligament progenitors. Development 141 2035 PMID: 24803652
Andäng et al (2008) Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation. Nature 451 460 PMID: 18185516
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We use DMSO for cryopreservation of HBECs - 92.5% of complete growth medium (containing DMEM/F12, 10% FBS, 1% penicillin-streptomycin and 40 µg/mL endothelial growth supplement) : 7.5% of DMSO. Cells grow well after thawing.
DMSO is used for dissolving CHIR (4423).Reconstitute 10 mg of CHIR in 2.149 ml of DMSO for a final concentration of 10mM.
Make 100µl aliquots and keep them at −80 °C for 6-12 months.