Discontinued Product

X-Gal (Cat. No. 5510) has been withdrawn from sale for commercial reasons.
Description: Used in cloning procedures with IPTG
Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
Purity: ≥98% (HPLC)
Reviews (1)

Biological Activity for X-Gal

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

Technical Data for X-Gal

M. Wt 408.63
Formula C14H15BrClNO6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 7240-90-6
PubChem ID 65181
Smiles ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for X-Gal

Certificate of Analysis / Product Datasheet
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References for X-Gal

References are publications that support the biological activity of the product.

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Keywords: X-Gal, X-Gal supplier, chromogenic, substrate, beta-galactosidase, β-galactosidase, b-galactosidase, Biochemicals, and, Molecular, Biology, 5510, Tocris Bioscience

Citations for X-Gal

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Currently there are no citations for X-Gal.

Reviews for X-Gal

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X gal for Blue White Screening.
By Anonymous on 02/04/2019
Species: Human

Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.

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