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Cat.No. 5510 - X-Gal | C14H15BrClNO6 | CAS No. 7240-90-6
Description: Used in cloning procedures with IPTG
Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
Purity: ≥98% (HPLC)
Reviews (1)
Literature (1)

Biological Activity

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

Technical Data

M. Wt 408.63
Formula C14H15BrClNO6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 7240-90-6
PubChem ID 65181
Smiles ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 40.86 100

Preparing Stock Solutions

The following data is based on the product molecular weight 408.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

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Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.45 mL 12.24 mL 24.47 mL
5 mM 0.49 mL 2.45 mL 4.89 mL
10 mM 0.24 mL 1.22 mL 2.45 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Keywords: X-Gal, X-Gal supplier, chromogenic, substrate, beta-galactosidase, β-galactosidase, b-galactosidase, Biochemicals, and, Molecular, Biology, 5510, Tocris Bioscience

Citations for X-Gal

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Reviews for X-Gal

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X gal for Blue White Screening.
By Anonymous on 02/04/2019
Species: Human

Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.

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