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X-Gal

Cat. No. 5510 Submit a Review Datasheet / COA / SDS
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Cat.No. 5510 - X-Gal | C14H15BrClNO6 | CAS No. 7240-90-6
Description: Used in cloning procedures with IPTG
Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
Purity: ≥98% (HPLC)
Datasheet
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies.

Technical Data

M. Wt 408.63
Formula C14H15BrClNO6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 7240-90-6
PubChem ID 65181
InChI Key OPIFSICVWOWJMJ-AEOCFKNESA-N
Smiles ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.86 100

Preparing Stock Solutions

The following data is based on the product molecular weight 408.63. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.45 mL 12.24 mL 24.47 mL
5 mM 0.49 mL 2.45 mL 4.89 mL
10 mM 0.24 mL 1.22 mL 2.45 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

Molarity Calculator

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References

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Keywords: X-Gal, X-Gal supplier, chromogenic, substrate, beta-galactosidase, β-galactosidase, b-galactosidase, Biochemicals, and, Molecular, Biology, 5510, Tocris Bioscience

Citations for X-Gal

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