X-Gal
Biological Activity
Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies.
Technical Data
| M. Wt | 408.63 |
| Formula | C14H15BrClNO6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 7240-90-6 |
| PubChem ID | 65181 |
| InChI Key | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| Smiles | ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 40.86 | 100 | |
| DMF | 20.43 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 408.63. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.45 mL | 12.24 mL | 24.47 mL |
| 5 mM | 0.49 mL | 2.45 mL | 4.89 mL |
| 10 mM | 0.24 mL | 1.22 mL | 2.45 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.49 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
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Keywords: chromogenic substrate beta-galactosidase β-galactosidase b-galactosidase Biochemicals and Molecular Biology
Citations for X-Gal
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Literature in this Area
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