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Description: Used in cloning procedures with IPTG
Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
Purity: ≥98% (HPLC)
Biological Activity
Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies.
Technical Data
| M. Wt | 408.63 |
| Formula | C14H15BrClNO6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 7240-90-6 |
| PubChem ID | 65181 |
| InChI Key | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| Smiles | ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 40.86 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 408.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.45 mL | 12.24 mL | 24.47 mL |
| 5 mM | 0.49 mL | 2.45 mL | 4.89 mL |
| 10 mM | 0.24 mL | 1.22 mL | 2.45 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.49 mL |
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References
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Keywords: X-Gal, X-Gal supplier, chromogenic, substrate, beta-galactosidase, β-galactosidase, b-galactosidase, Biochemicals, and, Molecular, Biology, 5510, Tocris Bioscience
Citations for X-Gal
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Reviews for X-Gal
Average Rating: 5 (Based on 1 Review.)
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X gal for Blue White Screening.
By
Anonymous on 02/04/2019
Species: Human
Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.
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