WS 6

Pricing Availability   Qty
Description: Induces growth and proliferation of pancreatic islet cells, murine totipotent stem cells, GABAergic neurons and hippocampal neurons
Chemical Name: 4-[[6-[(Cyclopropylcarbonyl)amino]-4-pyrimidinyl]oxy]-N-[4-[(4-methyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]benzeneacetamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (4)

Biological Activity for WS 6

WS 6 induces growth and proliferation of cultured islet α and β cells in vitro (EC50 = 0.28 μM at β cells). Inhibits growth of FLT3-ITD+ AML and K562 CML cells (IC50 values are <0.24 nM and 40.48 nM, respectively). Reduces blood glucose in an animal model of type 1 diabetes. In combination with TTNPB (Cat. No. 0761) and 1-azakenpaullone, WS 6 induces and maintains murine totipotent stem cells. In an animal model of depression WS 6 induces dendrite complexity, enhances hippocampal neurogenesis, increases GABAergic neurons and GABA expression, and displays antidepressant and anxiolytic activity. WS 6 is predicted to be a receptor tyrosine kinase inhibitor.

Technical Data for WS 6

M. Wt 568.59
Formula C29H31F3N6O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1421227-53-3
PubChem ID 71566751
InChI Key FTODTDQFHDJWIQ-UHFFFAOYSA-N
Smiles O=C(NC1=CC=C(C(=C1)C(F)(F)F)CN2CCN(C)CC2)CC3=CC=C(OC4=NC=NC(=C4)NC(=O)C5CC5)C=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WS 6

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 56.86 100
ethanol 56.86 100

Preparing Stock Solutions for WS 6

The following data is based on the product molecular weight 568.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.76 mL 8.79 mL 17.59 mL
5 mM 0.35 mL 1.76 mL 3.52 mL
10 mM 0.18 mL 0.88 mL 1.76 mL
50 mM 0.04 mL 0.18 mL 0.35 mL

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References for WS 6

References are publications that support the biological activity of the product.

Hu et al (2022) Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature PMID: 35728625

Zhang et al (2021) WS6 induces adult hippocampal neurogenesis in correlation to its antidepressant effect on the alleviation of depressive-like behaviors of rats. Neuroscience 473 119 PMID: 34455011

Menhaji-Klotz and Price (2015) Small molecules that promote regenerative repair for pancreatic and cardiovascular health. Bioorg.Med.Chem.Lett. 25 5465 PMID: 26508547

Shen et al (2013) Small-molecule inducer of β cell proliferation identified by high-throughput screening. J.Am.Chem.Soc. 135 1669 PMID: 23330637

Bregante et al (2021) Efficacy and synergy of small molecule inhibitors targeting FLT3-ITD+ acute myeloid leukemia. Cancers (Basel) 13 6181 PMID: 34944800


If you know of a relevant reference for WS 6, please let us know.

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Citations for WS 6

Citations are publications that use Tocris products.

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Reviews for WS 6

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

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Stem Cells Scientific Review

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

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Stem Cell Workflow Poster

Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.

Stem Cells Poster

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.