U0124

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Cat.No. 1868 - U0124 | C8H10N4S2 | CAS No. 108923-79-1
Description: Inactive analog of U0126 (Cat. No. 1144)
Chemical Name: Bis[amino(methylthio)methylene]butanedinitrile
Datasheet
Citations (5)
Literature
Pathways

Biological Activity

Inactive analog of U0126 (Cat. No. 1144). Used as a negative control. Does not inhibit MEK at concentrations up to 100 μM.

Technical Data

M. Wt 226.31
Formula C8H10N4S2
Storage Store at RT
CAS Number 108923-79-1
PubChem ID 5842955
InChI Key LBQNBMSPTURKGS-KQQUZDAGSA-N
Smiles N/C(SC)=C(\C(C#N)=C(SC)/N)/C#N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.63 100

Preparing Stock Solutions

The following data is based on the product molecular weight 226.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.42 mL 22.09 mL 44.19 mL
5 mM 0.88 mL 4.42 mL 8.84 mL
10 mM 0.44 mL 2.21 mL 4.42 mL
50 mM 0.09 mL 0.44 mL 0.88 mL

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Reconstitution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Favata et al (1998) Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J.Biol.Chem. 273 18623 PMID: 9660836

Fitzgerald (2000) Regulation of voltage-dependent calcium channels in rat sensory neurones involves a Ras-mitogen-activated protein kinase pathway. J.Physiol. 527 433 PMID: 10990531

Sekar et al (2001) Concerted transcriptional activation of the low density lipoprotein receptor gene by insulin and luteinizing hormone in cultured porcine granulosa-luteal cells: possible convergence of protein kinase A, phosphatidylinositol 3-kinase, and mitogen-activated Endocrinology 142 2921 PMID: 11416012


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Keywords: Mitogen-Activated Protein Kinase kinases MEK MEK

5 Citations for U0124

Citations are publications that use Tocris products. Selected citations for U0124 include:

Melemedjian et al (2011) Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol Pain 7 70 PMID: 21936900

Bentley et al (2011) Influence of chylomicron remnants on human monocyte activation in vitro. Skelet Muscle 21 871 PMID: 20674313

Kolber et al (2010) Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. J Neurosci 30 8203 PMID: 20554871

Melemedjian et al (2013) mTORC1 inhibition induces pain via IRS-1-dependent feedback activation of ERK. Pain 154 1080 PMID: 23607966

Philips et al (2013) MAPK establishes a molecular context that defines effective training patterns for long-term memory formation. Nutr Metab Cardiovasc Dis 33 7565 PMID: 23616561


Do you know of a great paper that uses U0124 from Tocris? If so please let us know.

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Reviews

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases
Pain

Pain Research Product Guide

A collection of over 250 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling
MAPK Signaling

MAPK Signaling Scientific Review

MAP kinase signaling is integral to the regulation of numerous cellular processes such as proliferation and differentiation, and as a result is an important focus of cancer and immunology research. Updated for 2016, this review discusses the regulation of the MAPK pathway and properties of MAPK cascades. Compounds available from Tocris are listed.

Pathways for U0124

MAPK

MAPK Signaling Pathway

The mitogen-activated protein kinase pathway evokes an intracellular signaling cascade in response to extracellular stimuli such as heat and stress. It can influence cell division, metabolism and survival.

Protocols

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