Inactive analog of U0126 (Cat. No. 1144). Used as a negative control. Does not inhibit MEK at concentrations up to 100 μM.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 226.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||4.42 mL||22.09 mL||44.19 mL|
|5 mM||0.88 mL||4.42 mL||8.84 mL|
|10 mM||0.44 mL||2.21 mL||4.42 mL|
|50 mM||0.09 mL||0.44 mL||0.88 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Favata et al (1998) Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J.Biol.Chem. 273 18623 PMID: 9660836
Fitzgerald (2000) Regulation of voltage-dependent calcium channels in rat sensory neurones involves a Ras-mitogen-activated protein kinase pathway. J.Physiol. 527 433 PMID: 10990531
Sekar et al (2001) Concerted transcriptional activation of the low density lipoprotein receptor gene by insulin and luteinizing hormone in cultured porcine granulosa-luteal cells: possible convergence of protein kinase A, phosphatidylinositol 3-kinase, and mitogen-activated Endocrinology 142 2921 PMID: 11416012
If you know of a relevant reference for U0124, please let us know.
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Keywords: Mitogen-Activated Protein Kinase kinases MEK MEK
5 Citations for U0124
Citations are publications that use Tocris products. Selected citations for U0124 include:
Melemedjian et al (2011) Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol Pain 7 70 PMID: 21936900
Bentley et al (2011) Influence of chylomicron remnants on human monocyte activation in vitro. Skelet Muscle 21 871 PMID: 20674313
Kolber et al (2010) Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. J Neurosci 30 8203 PMID: 20554871
Melemedjian et al (2013) mTORC1 inhibition induces pain via IRS-1-dependent feedback activation of ERK. Pain 154 1080 PMID: 23607966
Philips et al (2013) MAPK establishes a molecular context that defines effective training patterns for long-term memory formation. Nutr Metab Cardiovasc Dis 33 7565 PMID: 23616561
Do you know of a great paper that uses U0124 from Tocris? If so please let us know.
Literature in this Area
MAPK Signaling Scientific Review
MAP kinase signaling is integral to the regulation of numerous cellular processes such as proliferation and differentiation, and as a result is an important focus of cancer and immunology research. Updated for 2016, this review discusses the regulation of the MAPK pathway and properties of MAPK cascades. Compounds available from Tocris are listed.