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Description: Inactive analog of U0126 (Cat. No. 1144)
Chemical Name: Bis[amino(methylthio)methylene]butanedinitrile
Biological Activity
Inactive analog of U0126 (Cat. No. 1144). Does not inhibit MEK at concentrations up to 100 μM. Used as a negative control.
Active Analog also available.
Technical Data
| M. Wt | 226.31 |
| Formula | C8H10N4S2 |
| Storage | Store at RT |
| CAS Number | 108923-79-1 |
| PubChem ID | 5842955 |
| InChI Key | LBQNBMSPTURKGS-KQQUZDAGSA-N |
| Smiles | N/C(SC)=C(\C(C#N)=C(SC)/N)/C#N |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.63 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 226.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.42 mL | 22.09 mL | 44.19 mL |
| 5 mM | 0.88 mL | 4.42 mL | 8.84 mL |
| 10 mM | 0.44 mL | 2.21 mL | 4.42 mL |
| 50 mM | 0.09 mL | 0.44 mL | 0.88 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Favata et al (1998) Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J.Biol.Chem. 273 18623 PMID: 9660836
Fitzgerald (2000) Regulation of voltage-dependent calcium channels in rat sensory neurones involves a Ras-mitogen-activated protein kinase pathway. J.Physiol. 527 433 PMID: 10990531
Sekar et al (2001) Concerted transcriptional activation of the low density lipoprotein receptor gene by Ins and luteinizing hormone in cultured porcine granulosa-luteal cells: possible convergence of protein kinase A, phosphatidylinositol 3-kinase, and mitogen-activated pro Endocrinology 142 2921 PMID: 11416012
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Keywords: U0124, U0124 supplier, Mitogen-Activated, Protein, Kinase, kinases, MEK, 1868, Tocris Bioscience
9 Citations for U0124
Citations are publications that use Tocris products. Selected citations for U0124 include:
Bentley et al (2011) Influence of chylomicron remnants on human monocyte activation in vitro. Skelet Muscle 21 871 PMID: 20674313
Huang et al (2010) Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor. J Neurosci 30 6080 PMID: 20427666
Chen-Roetling et al (2009) Heme oxygenase activity and hemoglobin neurotoxicity are attenuated by inhibitors of the MEK/ERK pathway. Neuropharmacology 56 922 PMID: 19371583
Liverman et al (2009) Oestrogen increases nociception through ERK activation in the trigeminal ganglion: evidence for a peripheral mechanism of allodynia. Cephalalgia 29 520 PMID: 19210515
Kolber et al (2010) Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. J Neurosci 30 8203 PMID: 20554871
Melemedjian et al (2013) mTORC1 inhibition induces pain via IRS-1-dependent feedback activation of ERK. Pain 154 1080 PMID: 23607966
Ye et al (2012) Local synaptic integration of mitogen-activated protein kinase and protein kinase A signaling mediates intermediate-term synaptic facilitation in Aplysia. Proc Natl Acad Sci U S A 109 18162 PMID: 23071303
Philips et al (2013) MAPK establishes a molecular context that defines effective training patterns for long-term memory formation. Nutr Metab Cardiovasc Dis 33 7565 PMID: 23616561
Melemedjian et al (2011) Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol Pain 7 70 PMID: 21936900
Do you know of a great paper that uses U0124 from Tocris? Please let us know.
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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MAPK Signaling Scientific Review
MAP kinase signaling is integral to the regulation of numerous cellular processes such as proliferation and differentiation, and as a result is an important focus of cancer and immunology research. Updated for 2016, this review discusses the regulation of the MAPK pathway and properties of MAPK cascades. Compounds available from Tocris are listed.
Pathways for U0124
