TRIS hydrochloride

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Cat.No. 3164 - TRIS hydrochloride | C4H11NO3.HCl | CAS No. 1185-53-1
Description: Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Chemical Name: 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride
Purity: ≥99.9%
Reviews (1)

Biological Activity

Commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS) in combination with NaCl (Cat. No. 3146), Tris-acetate-ETDA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.

Technical Data

M. Wt 157.6
Formula C4H11NO3.HCl
Storage Store at RT
Purity ≥99.9%
CAS Number 1185-53-1
PubChem ID 93573
Smiles OCC(CO)(N)CO.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 630.4 4000

Preparing Stock Solutions

The following data is based on the product molecular weight 157.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
40 mM 0.16 mL 0.79 mL 1.59 mL
200 mM 0.03 mL 0.16 mL 0.32 mL
400 mM 0.02 mL 0.08 mL 0.16 mL
2000 mM 0 mL 0.02 mL 0.03 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Citations for TRIS hydrochloride

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Western blot.
By Anonymous on 07/28/2018
Assay Type: In Vitro
Species: Other

Used to prepare gels for western blot electrophoresis.

It is easier to adjust pH with this product compared to tris base (3163)