Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewTPPB
Description: High affinity PKC activator; also APP modulator
Chemical Name: (2E,4E)-N-[(2S,5S)-1,2,3,4,5,6-Hexahydro-5-(hydroxymethyl)-1-methyl-2-(1-methylethyl)-3-oxo-1,4-benzodiazocin-8-yl]-5-[4-(trifluoromethyl)phenyl]-2,4-pentadienamide
Purity: ≥98% (HPLC)
Biological Activity for TPPB
TPPB is a high affinity and cell permeable protein kinase C activator (Ki =11.9 nM), which induces differentiation of hESCs into Pdx-1 expressing pancreatic progenitor cells. TPPB also enhances the non-amyloidgenic processing of amyloid precursor protein, increasing secretion of sAPPα.
Technical Data for TPPB
| M. Wt | 501.54 |
| Formula | C27H30F3N3O3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 497259-23-1 |
| PubChem ID | 9935767 |
| InChI Key | WOLVEMPZUIFSII-IHHOKICGSA-N |
| Smiles | CC([C@@H]1N(C2=CC=C(C=C2C[C@H](NC1=O)CO)NC(/C=C/C=C/C3=CC=C(C(F)(F)F)C=C3)=O)C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for TPPB
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.08 | 50 |
Preparing Stock Solutions for TPPB
The following data is based on the product molecular weight 501.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.99 mL | 19.94 mL | 39.88 mL |
| 2.5 mM | 0.8 mL | 3.99 mL | 7.98 mL |
| 5 mM | 0.4 mL | 1.99 mL | 3.99 mL |
| 25 mM | 0.08 mL | 0.4 mL | 0.8 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for TPPB
References for TPPB
References are publications that support the biological activity of the product.
Rezania et al (2014) Reversal of diabetes with Ins-producing cells derived in vitro from human pluripotent stem cells. Nat.Biotechnol. 32 1121 PMID: 25211370
Chen et al (2009) A small molecule that directs differentiation of human ESCs into the pancreatic lineage. Nat.Chem.Biol. 5 258 PMID: 19287398
Kozikowski et al (2003) New amide-bearing benzolactam-based protein kinase C modulators induce enhanced secretion of the amyloid precursor protein metabolite sAPPα. J.Med.Chem. 46 364 PMID: 12540236
If you know of a relevant reference for TPPB, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: TPPB, TPPB supplier, PKC, activator, high, affinity, induces, differentiation, Pdx-1, pancreatic, progenitor, amyloid, precursor, protein, cell, permeable, APP, stem, cells, Protein, Kinase, C, Other, Differentiation, Products, Amyloid, Beta, Peptides, 5343, Tocris Bioscience
6 Citations for TPPB
Citations are publications that use Tocris products. Selected citations for TPPB include:
Kristina G et al (2021) Generation of insulin-producing pancreatic β cells from multiple human stem cell lines. Nat Protoc 16 4109-4143 PMID: 34349281
Robert G et al (2022) Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians. Cells 11 PMID: 35563754
Yang et al (2020) A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell 27 125 PMID: 32579880
Rudolf et al (2020) Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction. Cell Rep Med 1 100097 PMID: 33205073
Kenichiro et al (2020) Bioinformatic Analyses of miRNA-mRNA Signature during hiPSC Differentiation towards Insulin-Producing Cells upon HNF4α Mutation. Biomedicines 8 PMID: 32605028
Leonardo et al (2020) Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nat Biotechnol 38 460-470 PMID: 32094658
Do you know of a great paper that uses TPPB from Tocris? Please let us know.
Reviews for TPPB
There are currently no reviews for this product. Be the first to review TPPB and earn rewards!
Have you used TPPB?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cancer Research Product Guide
A collection of over 750 products for cancer research, the guide includes research tools for the study of:
- Cancer Metabolism
- Epigenetics in Cancer
- Receptor Signaling
- Cell Cycle and DNA Damage Repair
- Angiogenesis
- Invasion and Metastasis