Pricing Availability   Qty
Description: High affinity PKC activator; also APP modulator
Chemical Name: (2E,4E)-N-[(2S,5S)-1,2,3,4,5,6-Hexahydro-5-(hydroxymethyl)-1-methyl-2-(1-methylethyl)-3-oxo-1,4-benzodiazocin-8-yl]-5-[4-(trifluoromethyl)phenyl]-2,4-pentadienamide
Purity: ≥98% (HPLC)
Citations (6)

Biological Activity for TPPB

TPPB is a high affinity and cell permeable protein kinase C activator (Ki =11.9 nM), which induces differentiation of hESCs into Pdx-1 expressing pancreatic progenitor cells. TPPB also enhances the non-amyloidgenic processing of amyloid precursor protein, increasing secretion of sAPPα.

Technical Data for TPPB

M. Wt 501.54
Formula C27H30F3N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 497259-23-1
PubChem ID 9935767
Smiles CC([C@@H]1N(C2=CC=C(C=C2C[C@H](NC1=O)CO)NC(/C=C/C=C/C3=CC=C(C(F)(F)F)C=C3)=O)C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TPPB

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 25.08 50

Preparing Stock Solutions for TPPB

The following data is based on the product molecular weight 501.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.99 mL 19.94 mL 39.88 mL
2.5 mM 0.8 mL 3.99 mL 7.98 mL
5 mM 0.4 mL 1.99 mL 3.99 mL
25 mM 0.08 mL 0.4 mL 0.8 mL

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Product Datasheets for TPPB

Certificate of Analysis / Product Datasheet
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References for TPPB

References are publications that support the biological activity of the product.

Rezania et al (2014) Reversal of diabetes with Ins-producing cells derived in vitro from human pluripotent stem cells. Nat.Biotechnol. 32 1121 PMID: 25211370

Chen et al (2009) A small molecule that directs differentiation of human ESCs into the pancreatic lineage. Nat.Chem.Biol. 5 258 PMID: 19287398

Kozikowski et al (2003) New amide-bearing benzolactam-based protein kinase C modulators induce enhanced secretion of the amyloid precursor protein metabolite sAPPα. J.Med.Chem. 46 364 PMID: 12540236

If you know of a relevant reference for TPPB, please let us know.

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6 Citations for TPPB

Citations are publications that use Tocris products. Selected citations for TPPB include:

Yang et al (2020) A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell 27 125 PMID: 32579880

Kristina G et al (2021) Generation of insulin-producing pancreatic β cells from multiple human stem cell lines. Nat Protoc 16 4109-4143 PMID: 34349281

Robert G et al (2022) Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians. Cells 11 PMID: 35563754

Rudolf et al (2020) Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction. Cell Rep Med 1 100097 PMID: 33205073

Do you know of a great paper that uses TPPB from Tocris? Please let us know.

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