Torin 2

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Cat.No. 4248 - Torin 2 | C24H15F3N4O | CAS No. 1223001-51-1
Description: Potent and selective mTOR inhibitor
Chemical Name: 9-(6-Amino-3-pyridinyl)-1-[3-(trifluoromethyl)phenyl]-benzo[h]-1,6-naphthyridin-2(1H)-one
Purity: ≥98% (HPLC)
Datasheet
Citations (9)
Literature
Pathways

Biological Activity

Potent and selective mTOR inhibitor (IC50 = 2.1 nM). Displays 800-fold cellular selectivity for mTOR over PI3K (cellular EC50 values are 0.25 and 200 nM for mTOR and PI3K respectively). Activates autophagy. Orally available.

Licensing Information

Sold under license from Whitehead Institute for Biomedical Research.

Compound Libraries

Torin 2 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox II. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 432.4
Formula C24H15F3N4O
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1223001-51-1
PubChem ID 51358113
InChI Key GUXXEUUYCAYESJ-UHFFFAOYSA-N
Smiles O=C(C=C3)N(C5=CC=CC(C(F)(F)F)=C5)C2=C3C=NC1=CC=C(C4=CC=C(N)N=C4)C=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 8.65 20

Preparing Stock Solutions

The following data is based on the product molecular weight 432.4. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.31 mL 11.56 mL 23.13 mL
5 mM 0.46 mL 2.31 mL 4.63 mL
10 mM 0.23 mL 1.16 mL 2.31 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Liu et al (2011) Discovery of 9-(6-aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one (Torin2) as a potent, selective, and orally available mammalian target of rapamycin (mTOR) inhibitor for treatment of cancer. J.Med.Chem. 54 1473 PMID: 21322566

Simioni et al (2014) Activity of the novel mTOR inhibitor Torin-2 in B-precursor acute lymphoblastic leukemia and its therapeutic potential to prevent Akt reactivation. Oncotarget 30 10034 PMID: 25296981

Galluzzi et al (2017) Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. PMID: 8529316


If you know of a relevant reference for Torin 2, please let us know.

View Related Products by Product Action

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Keywords: Torin2 mtor mammalian target rapamycin selective inhibitors inhibits mTOR

9 Citations for Torin 2

Citations are publications that use Tocris products. Selected citations for Torin 2 include:

Hussain et al (2015) Dual Targeting of mTOR Activity with Torin2 Potentiates Anticancer Effects of Cisplatin in Epithelial Ovarian Cancer. Mol Med 21 466 PMID: 26023849

Toops et al (2015) Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium. Nat Commun 26 41640 PMID: 25378587

Yu et al (2015) PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. BMC Biochem 6 7212 PMID: 25998442

Han et al (2014) Parallel measurement of dynamic changes in translation rates in single cells. Nat Methods 11 86 PMID: 24213167

Ahmed et al (2014) High prevalence of mTOR complex activity can be targeted using Torin2 in papillary thyroid carcinoma. Carcinogenesis 35 1564 PMID: 24583924

Zullo et al (2014) Mammalian target of Rapamycin inhibition and mycobacterial survival are uncoupled in murine macrophages. Cell 15 4 PMID: 24528777

Brown et al (2014) Mitogen-activated protein kinase-interacting kinase regulates mTOR/AKT signaling and controls the serine/arginine-rich protein kinase-responsive type 1 internal ribosome entry site-mediated translation and viral oncolysis. Mol Biol Cell 88 13149 PMID: 25187540

Hwang et al (2013) Protein phosphatase 2A isoforms utilizing Aβ scaffolds regulate differentiation through control of Akt protein. J Biol Chem 288 32064 PMID: 24052256

Montané and Menand (2013) ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change. J Virol 64 4361 PMID: 23963679


Do you know of a great paper that uses Torin 2 from Tocris? If so please let us know.

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Pathways for Torin 2

mTOR

mTOR Signaling Pathway

mTOR is a serine/threonine kinase that nucleates at multiprotein complexes mTORC1 and mTORC2. Signaling by these complexes regulates cell growth, proliferation and survival.

Protocols

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