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Fluorescent form of AM 251. AM 251 conjugated with 5-carboxytetramethylrhodamine (5-TAMRA) that fluoresces at 543 nm excitation (590 nm emission). Displays GPR55 binding activity.
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|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 974.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||10.26 mL||51.28 mL||102.57 mL|
|0.5 mM||2.05 mL||10.26 mL||20.51 mL|
|1 mM||1.03 mL||5.13 mL||10.26 mL|
|5 mM||0.21 mL||1.03 mL||2.05 mL|
References are publications that support the biological activity of the product.
Daly et al (2010) Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteries. Br.J.Pharmacol. 159 787 PMID: 20136833
Daly et al (2008) Visualisation of vascular cannabinoid receptors and their potential interaction with α1-adrenergic receptors. Vascular and Smooth Muscle Phys.Soc. Symposium
Sylantyev et al (2013) Cannabinoid- and lysophosphatidylinositol-sensitive receptor GPR55 boosts neurotransmitter release at central synapses. Proc.Natl.Acad.Sci.U.S.A. 110 5193 PMID: 23472002
Bruno et al (2014) Beyond radio-displacement techniques for identification of CB1 ligands: the first application of a fluorescence-quenching assay. Sci.Rep. 4 3757 PMID: 24441508
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Keywords: Tocrifluor T1117, Tocrifluor T1117 supplier, fluorescent, CB1, receptor, ligands, Fluorescent, Probes, cannabinoids, Receptors, cb1r, cb2r, Receptor, GPR55, 2540, Tocris Bioscience
3 Citations for Tocrifluor T1117
Citations are publications that use Tocris products. Selected citations for Tocrifluor T1117 include:
Daly et al (2010) Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteries. Br J Pharmacol 159 787 PMID: 20136833
Stornaiuolo et al (2015) Endogenous vs Exogenous Allosteric Modulators in GPCRs: A dispute for shuttling CB1 among different membrane microenvironments. Sci Rep 5 15453 PMID: 26482099
Tortoriello et al (2013) Targeted lipidomics in Drosophila melanogaster identifies novel 2-monoacylglycerols and N-acyl amides. PLoS One 8 e67865 PMID: 23874457
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Literature in this Area
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