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Submit ReviewSynaptoRedTM C2 is a fluorescent dye; stains synaptic vesicles. Becomes fluorescent when incorporated into plasma membrane, used to follow synaptic activity at the synapse or neuromuscular junction. Excitation and emission spectra are ~ 515 and 640 nm respectively.
SynaptoRed is a trademark of Biotium, Inc.
M. Wt | 607.51 |
Formula | C30H45Br2N3 |
Storage | Store at -20°C |
Purity | ≥95% (HPLC) |
CAS Number | 162112-35-8 |
PubChem ID | 2733635 |
InChI Key | AFVSZGYRRUMOFH-UHFFFAOYSA-L |
Smiles | CCN(CC)C(C=C2)=CC=C2\C=C/C=C/C=C\C1=CC=[N+](CCC[N+](CC)(CC)CC)C=C1.[Br-].[Br-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 6.08 | 10 |
The following data is based on the product molecular weight 607.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 16.46 mL | 82.3 mL | 164.61 mL |
0.5 mM | 3.29 mL | 16.46 mL | 32.92 mL |
1 mM | 1.65 mL | 8.23 mL | 16.46 mL |
5 mM | 0.33 mL | 1.65 mL | 3.29 mL |
References are publications that support the biological activity of the product.
Daniel et al (2012) Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat.Protoc. 7 1439 PMID: 22767087
Fischer-Parton et al (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J.Microsc. 198 246 PMID: 10849201
Dason et al (2010) Vesicular sterols are essential for synaptic vesicle cycling. J.Neurosci. 30 15856 PMID: 21106824
If you know of a relevant reference for SynaptoRedTM C2, please let us know.
Keywords: SynaptoRed C2, SynaptoRed C2 supplier, Fluorescent, probe, FM4-64, excitation, wavelength, emission, synaptic, vesicles, Organelle, and, Cell, Structure, Stains, Histology, Neuron, Astrocyte, Probes, Lipid, Membrane, 5118, Tocris Bioscience
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Cultured cortical neurons labeled with 5 micromolar SynaptoRed for 5 minutes upon KCl depolarization and washing with aCSF.
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: