SynaptoRedTM C2

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Cat.No. 5118 - SynaptoRed C2 | C30H45Br2N3 | CAS No. 162112-35-8
Description: Fluorescent dye; stains synaptic vesicles
Alternative Names: FM4-64
Chemical Name: 4-[6-[4-(Diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Purity: ≥95% (HPLC)

Biological Activity

Fluorescent dye; stains synaptic vesicles. Becomes fluorescent when incorporated into plasma membrane, used to follow synaptic activity at the synapse or neuromuscular junction. Excitation and emission spectra are ~ 515 and 640 nm respectively.

Licensing Information

SynaptoRed is a trademark of Biotium, Inc.

Technical Data

M. Wt 607.51
Formula C30H45Br2N3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 162112-35-8
PubChem ID 2733635
Smiles CCN(CC)C(C=C2)=CC=C2\C=C/C=C/C=C\C1=CC=[N+](CCC[N+](CC)(CC)CC)C=C1.[Br-].[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 6.08 10

Preparing Stock Solutions

The following data is based on the product molecular weight 607.51. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.65 mL 8.23 mL 16.46 mL
5 mM 0.33 mL 1.65 mL 3.29 mL
10 mM 0.16 mL 0.82 mL 1.65 mL
50 mM 0.03 mL 0.16 mL 0.33 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Daniel et al (2012) Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat.Protoc. 7 1439 PMID: 22767087

Fischer-Parton et al (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J.Microsc. 198 246 PMID: 10849201

Dason et al (2010) Vesicular sterols are essential for synaptic vesicle cycling. J.Neurosci. 30 15856 PMID: 1106824

If you know of a relevant reference for SynaptoRedTM C2, please let us know.

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