SynaptoRedTM C2

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Description: Fluorescent dye; stains synaptic vesicles
Alternative Names: FM4-64
Chemical Name: 4-[6-[4-(Diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Purity: ≥95% (HPLC)
Citations (1)
Reviews (2)
Literature (1)

Biological Activity for SynaptoRedTM C2

SynaptoRedTM C2 is a fluorescent dye; stains synaptic vesicles. Becomes fluorescent when incorporated into plasma membrane, used to follow synaptic activity at the synapse or neuromuscular junction. Excitation and emission spectra are ~ 515 and 640 nm respectively.

Licensing Information

SynaptoRed is a trademark of Biotium, Inc.

Technical Data for SynaptoRedTM C2

M. Wt 607.51
Formula C30H45Br2N3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 162112-35-8
PubChem ID 2733635
Smiles CCN(CC)C(C=C2)=CC=C2\C=C/C=C/C=C\C1=CC=[N+](CCC[N+](CC)(CC)CC)C=C1.[Br-].[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SynaptoRedTM C2

Solvent Max Conc. mg/mL Max Conc. mM
water 10 17

Preparing Stock Solutions for SynaptoRedTM C2

The following data is based on the product molecular weight 607.51. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.17 mM 9.68 mL 48.41 mL 96.83 mL
0.85 mM 1.94 mL 9.68 mL 19.37 mL
1.7 mM 0.97 mL 4.84 mL 9.68 mL
8.5 mM 0.19 mL 0.97 mL 1.94 mL

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Product Datasheets for SynaptoRedTM C2

Certificate of Analysis / Product Datasheet
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References for SynaptoRedTM C2

References are publications that support the biological activity of the product.

Daniel et al (2012) Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat.Protoc. 7 1439 PMID: 22767087

Fischer-Parton et al (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J.Microsc. 198 246 PMID: 10849201

Dason et al (2010) Vesicular sterols are essential for synaptic vesicle cycling. J.Neurosci. 30 15856 PMID: 21106824

If you know of a relevant reference for SynaptoRedTM C2, please let us know.

Keywords: SynaptoRed C2, SynaptoRed C2 supplier, Fluorescent, probe, FM4-64, excitation, wavelength, emission, synaptic, vesicles, Organelle, and, Cell, Structure, Stains, Histology, Neuron, Astrocyte, Probes, Lipid, Membrane, 5118, Tocris Bioscience

1 Citation for SynaptoRedTM C2

Citations are publications that use Tocris products. Selected citations for SynaptoRedTM C2 include:

Richard et al (2021) Plant cell adhesion and growth on artificial fibrous scaffolds as an in vitro model for plant development. Sci Adv 7 eabj1469 PMID: 34669469

Do you know of a great paper that uses SynaptoRedTM C2 from Tocris? Please let us know.

Reviews for SynaptoRedTM C2

Average Rating: 4 (Based on 2 Reviews.)

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synaptic vesicle dye.
By Karthik Krishnamurthy on 08/03/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: cortical neurons

Cultured cortical neurons labeled with 5 micromolar SynaptoRed for 5 minutes upon KCl depolarization and washing with aCSF.

review image

synaptic vesicle release.
By Anonymous on 12/12/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons

for synaptic vesicle recycling studies in cortical and motor neurons

10 micromolar of synaptored was used

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

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