SynaptoRedTM C2

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Cat.No. 5118 - SynaptoRed C2 | C30H45Br2N3 | CAS No. 162112-35-8
Description: Fluorescent dye; stains synaptic vesicles
Alternative Names: FM4-64
Chemical Name: 4-[6-[4-(Diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews (1)
Literature

Biological Activity

Fluorescent dye; stains synaptic vesicles. Becomes fluorescent when incorporated into plasma membrane, used to follow synaptic activity at the synapse or neuromuscular junction. Excitation and emission spectra are ~ 515 and 640 nm respectively.

Licensing Information

SynaptoRed is a trademark of Biotium, Inc.

Technical Data

M. Wt 607.51
Formula C30H45Br2N3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 162112-35-8
PubChem ID 2733635
InChI Key AFVSZGYRRUMOFH-UHFFFAOYSA-L
Smiles CCN(CC)C(C=C2)=CC=C2\C=C/C=C/C=C\C1=CC=[N+](CCC[N+](CC)(CC)CC)C=C1.[Br-].[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.08 10

Preparing Stock Solutions

The following data is based on the product molecular weight 607.51. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.65 mL 8.23 mL 16.46 mL
5 mM 0.33 mL 1.65 mL 3.29 mL
10 mM 0.16 mL 0.82 mL 1.65 mL
50 mM 0.03 mL 0.16 mL 0.33 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Daniel et al (2012) Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat.Protoc. 7 1439 PMID: 22767087

Fischer-Parton et al (2000) Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J.Microsc. 198 246 PMID: 10849201

Dason et al (2010) Vesicular sterols are essential for synaptic vesicle cycling. J.Neurosci. 30 15856 PMID: 21106824


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Keywords: SynaptoRed C2, supplier, Fluorescent, probe, FM4-64, excitation, wavelength, emission, synaptic, vesicles, FM4-64, Fluorescent, Probes, Fluorescent, Probes, Tocris Bioscience

Citations for SynaptoRedTM C2

Citations are publications that use Tocris products.

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Reviews for SynaptoRedTM C2

Average Rating: 4 (Based on 1 Review)

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synaptic vesicle release
By Anonymous on 12/12/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons

for synaptic vesicle recycling studies in cortical and motor neurons

10 micromolar of synaptored was used

review image

Literature in this Area

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