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Description: Inhibitor of NF-κB activation; also inhibits SXC and folate transporter 1
Chemical Name: 5-[[4-(2-Pyridylsulfamoyl)phenyl]azo]salicylic acid
Purity: ≥97% (HPLC)
Citations (3)
Pathways (1)

Biological Activity for Sulfasalazine

Sulfasalazine is an inhibitor of NF-κB activation. Inhibits in vitro growth of the human pancreatic cancer cell lines MIA PaCa-2 and PANC-1, and induces apoptosis in glioblastoma cell lines. Also inhibits the cystine-glutamate antiporter, system Xc (SXC). Inhibitor of folate transporter 1 (SLC19A1); inhibits cellular uptake of Methotrexate (Cat. No. 1230) and cyclic dinucleotides, including 2',3'-cGAMP (Cat.No. 5945). Anti-inflammatory. Induces ferroptosis.

Compound Libraries for Sulfasalazine

Sulfasalazine is also offered as part of the Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for Sulfasalazine

M. Wt 398.39
Formula C18H14N4O5S
Storage Store at RT
Purity ≥97% (HPLC)
CAS Number 599-79-1
PubChem ID 5359476
Smiles OC1=CC=C(/N=N/C2=CC=C(S(NC3=NC=CC=C3)(=O)=O)C=C2)C=C1C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Sulfasalazine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 39.84 100

Preparing Stock Solutions for Sulfasalazine

The following data is based on the product molecular weight 398.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.51 mL 12.55 mL 25.1 mL
5 mM 0.5 mL 2.51 mL 5.02 mL
10 mM 0.25 mL 1.26 mL 2.51 mL
50 mM 0.05 mL 0.25 mL 0.5 mL

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Product Datasheets for Sulfasalazine

Certificate of Analysis / Product Datasheet
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References for Sulfasalazine

References are publications that support the biological activity of the product.

Robe et al (2004) In vitro and in vivo activity of the nuclear factor-κB inhbitor sulfasal. in human glioblastomas. Clin.Cancer Res. 10 5595 PMID: 15328202

Lo et al (2010) Potential use fo the anti-inflammatory drug, sulfasalazine, for targeted therapy of pancreatic cancer. Curr.Oncol. 17 9 PMID: 20567622

Sontheimer and Bridges (2012) Sulfasalazine for brain cancer fits. Expert Opin.Invest.Drugs 21 575 PMID: 22404218

Jansen et al (2004) Sulfasalazine is a potent inhibitor of the reduced folate carrier: implications for combination therapies with methotrexate in rheumatoid arthritis. Arthritis Rheum. 50 2130 PMID: 15248210

Luteijn et al (2019) SLC19A1 transports immunoreactive cyclic dinucleotides. Nature 573 434 PMID: 31511694

Xie et al (2016) Ferroptosis: process and function. Cell Death Differ. 23 369 PMID: 26794443

If you know of a relevant reference for Sulfasalazine, please let us know.

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Keywords: Sulfasalazine, Sulfasalazine supplier, antirheumatic, antiinflammatories, antiinflammatory, arthritis, system, XC, SXC, cysteine-glutamate, antiporters, NF-kappaB, NF-kB, NF-κB, inhibitors, inhibits, ferroptosis, inducers, induces, folate, transporter, 1, cGAMP, SLC19A1, xCT, SLC11A7, NF-kB/IkB, Ferroptosis, Other, Ion, Pumps/Transporters, 4935, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Sulfasalazine (Salicylazosulfapyridine), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to

3 Citations for Sulfasalazine

Citations are publications that use Tocris products. Selected citations for Sulfasalazine include:

Shin et al (2018) Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo. Oncogenesis 7 86 PMID: 30425240

Yeligar et al (2009) Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1. J Immunol 183 5964 PMID: 19828633

Christopher T et al (2017) In vitro modeling of HIV proviral activity in microglia. FEBS J 284 4096-4114 PMID: 29114997

Do you know of a great paper that uses Sulfasalazine from Tocris? Please let us know.

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Pathways for Sulfasalazine

NF-κB Signaling Pathway

NF-κB Signaling Pathway

NF-κB signaling plays an important role in inflammation, the innate and adaptive immune response and stress. Dysregulated signaling can occur in inflammatory and autoimmune diseases.