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SMCy 5.5 is a fluorogenic fluorescent probe for imaging lipid droplets that exhibits sharp excitation and emission bands and high brightness. SMCy 5.5 is suitable for use with two-photon microscopy. It is useful for deep tissue imaging and 3D imaging. Excitation/emission maxima for SMCy 5.5 in THF are 643/672 nm; Quantum yield = 0.75; Extinction coefficient = 209,000 M-1 cm-1. SMCy 5.5 displays high two-photon absoprtion (TPA) cross-section values (13330 GM and 10400 GM at 770 and 820 nm, respectively). The probe also has a click reactive handle.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 693.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||14.42 mL||72.08 mL||144.17 mL|
|0.5 mM||2.88 mL||14.42 mL||28.83 mL|
|1 mM||1.44 mL||7.21 mL||14.42 mL|
|5 mM||0.29 mL||1.44 mL||2.88 mL|
References are publications that support the biological activity of the product.
Collot et al (2018) Ultrabright and fluorogenic probes for multicolor imaging and tracking of lipid droplets in cells and tissues. J.Am.Chem.Soc. 140 5401 PMID: 29446627
Fam et al (2018) Recent advances in fluorescent probes for lipid droplets. Materials (Basel) 11 1768 PMID: 30231571
If you know of a relevant reference for SMCy 5.5, please let us know.
Keywords: SMCy 5.5, SMCy 5.5 supplier, Fluorescent, fluorogenic, probe, imaging, lipid, droplets, two-photon, microscopy, 2-photon, Lipid, Probes, and, Cell, Membrane, Stains, Click-Reactive, Dyes, 7295, Tocris Bioscience
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: