Pricing Availability Delivery Time Qty
Cat.No. 3617 - SANT-2 | C26H26ClN3O4 | CAS No. 329196-48-7
Description: Inhibitor of Hedgehog (Hh) signaling; antagonizes smoothened activity
Chemical Name: N-[3-(1H-Benzimidazol-2-yl)-4-chlorophenyl]-3,4,5-triethoxybenzamide
Purity: ≥98% (HPLC)

Biological Activity

Inhibitor of Sonic hedgehog (Shh) signaling; antagonizes smoothened receptor activity (KD = 12 nM). Displays allosteric binding characteristics similar to SANT-1 (Cat. No. 1974). Displaces smo-[3H]SAG-1.3 and -[3H]Cyclopamine binding (Ki values are 7.8 nM and 8.4 nM respectively).

Compound Libraries

SANT-2 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 479.96
Formula C26H26ClN3O4
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 329196-48-7
PubChem ID 1367095
Smiles ClC1=CC=C(NC(C4=CC(OCC)=C(OCC)C(OCC)=C4)=O)C=C1C3=NC2=CC=CC=C2N3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 48 100
ethanol 48 100

Preparing Stock Solutions

The following data is based on the product molecular weight 479.96. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.08 mL 10.42 mL 20.84 mL
5 mM 0.42 mL 2.08 mL 4.17 mL
10 mM 0.21 mL 1.04 mL 2.08 mL
50 mM 0.04 mL 0.21 mL 0.42 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Rominger et al (2009) Evidence for allosteric interactions of antagonist binding to the smoothened receptor. J.Pharmacol.Exp.Ther. 329 995 PMID: 19304771

Chen et al (2002) Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci. 99 14071 PMID:

If you know of a relevant reference for SANT-2, please let us know.

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Citations for SANT-2

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Literature in this Area


Cancer Research Product Guide

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Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for SANT-2


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