Rose Bengal

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Cat.No. 5168 - Rose Bengal | C20H2Cl4I4Na2O5 | CAS No. 632-69-9
Description: Stains exposed epithelium in eye. Also VGlut and VMAT inhibitor
Chemical Name: 4,5,6,7-Tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one disodium salt
Purity: ≥98% (HPLC)
Citations (1)
Literature (1)

Biological Activity

Potent VGlut and vesicular monoamine transporter (VMAT) inhibitor (Ki values are 19 and 64 nM, respectively). Inhibits ATP dependent vesicular glutamate uptake (IC50 = 37 nM) and diminishes the amount of exocytotically released glutamate in synaptosomes. Decreases synaptic transmission in the dentate gyrus. Also stains exposed epithelium in the eye. Cell permeable.

Technical Data

M. Wt 1017.64
Formula C20H2Cl4I4Na2O5
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 632-69-9
PubChem ID 32343
Smiles O=C2C(I)=CC(C(OC3=C(I)C(O[Na])=C(I)C=C34)=C2I)=C4C1=C(C(O[Na])=O)C(Cl)=C(Cl)C(Cl)=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 101.76 100
DMSO 50.88 50

Preparing Stock Solutions

The following data is based on the product molecular weight 1017.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.98 mL 4.91 mL 9.83 mL
5 mM 0.2 mL 0.98 mL 1.97 mL
10 mM 0.1 mL 0.49 mL 0.98 mL
50 mM 0.02 mL 0.1 mL 0.2 mL

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References are publications that support the biological activity of the product.

Pietrancosta et al (2010) Rose Bengal analogs and vesicular glutamate transporters (VGLUTs). Bioorg.Med.Chem. 18 6922 PMID: 20708942

Ogita et al (2001) Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal. J Neurochem. 77 34 PMID: 11279259

Neale et al (2013) Modulation of hippocampal synaptic transmission by the kynurenine pathway member xanthurenic acid and other VGLUT inhibitors. Neuropsychopharmacology. Neuropsychopharmacology 38 1060 PMID: 23303071

Feenstra et al (1992) What is actually stained by rose bengal? Arch.Ophthalmol. 110 984 PMID: 1637285

If you know of a relevant reference for Rose Bengal, please let us know.

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1 Citation for Rose Bengal

Citations are publications that use Tocris products. Selected citations for Rose Bengal include:

Ahmad et al (2019) Perivascular Adipocytes Store Norepinephrine by Vesicular Transport. Arterioscler Thromb Vasc Biol 39 188 PMID: 30567483

Do you know of a great paper that uses Rose Bengal from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Probes and Dyes

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