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Submit ReviewRose Bengal
Description: Stains exposed epithelium in eye. Also VGlut and VMAT inhibitor
Chemical Name: 4,5,6,7-Tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one disodium salt
Purity: ≥98% (HPLC)
Biological Activity for Rose Bengal
Rose Bengal is a potent VGlut and vesicular monoamine transporter (VMAT) inhibitor (Ki values are 19 and 64 nM, respectively). Inhibits ATP dependent vesicular glutamate uptake (IC50 = 37 nM) and diminishes the amount of exocytotically released glutamate in synaptosomes. Decreases synaptic transmission in the dentate gyrus. Also stains exposed epithelium in the eye. Cell permeable.
Technical Data for Rose Bengal
| M. Wt | 1017.64 |
| Formula | C20H2Cl4I4Na2O5 |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 632-69-9 |
| PubChem ID | 32343 |
| InChI Key | UWBXIFCTIZXXLS-UHFFFAOYSA-L |
| Smiles | O=C2C(I)=CC(C(OC3=C(I)C(O[Na])=C(I)C=C34)=C2I)=C4C1=C(C(O[Na])=O)C(Cl)=C(Cl)C(Cl)=C1Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Rose Bengal
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 101.76 | 100 | |
| DMSO | 50.88 | 50 |
Preparing Stock Solutions for Rose Bengal
The following data is based on the product molecular weight 1017.64. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.98 mL | 4.91 mL | 9.83 mL |
| 5 mM | 0.2 mL | 0.98 mL | 1.97 mL |
| 10 mM | 0.1 mL | 0.49 mL | 0.98 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.2 mL |
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Product Datasheets for Rose Bengal
References for Rose Bengal
References are publications that support the biological activity of the product.
Pietrancosta et al (2010) Rose Bengal analogs and vesicular glutamate transporters (VGLUTs). Bioorg.Med.Chem. 18 6922 PMID: 20708942
Ogita et al (2001) Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal. J Neurochem. 77 34 PMID: 11279259
Neale et al (2013) Modulation of hippocampal synaptic transmission by the kynurenine pathway member xanthurenic acid and other VGLUT inhibitors. Neuropsychopharmacology. Neuropsychopharmacology 38 1060 PMID: 23303071
Feenstra et al (1992) What is actually stained by rose bengal? Arch.Ophthalmol. 110 984 PMID: 1637285
If you know of a relevant reference for Rose Bengal, please let us know.
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1 Citation for Rose Bengal
Citations are publications that use Tocris products. Selected citations for Rose Bengal include:
Ahmad et al (2019) Perivascular Adipocytes Store Norepinephrine by Vesicular Transport. Arterioscler Thromb Vasc Biol 39 188 PMID: 30567483
Do you know of a great paper that uses Rose Bengal from Tocris? Please let us know.
Reviews for Rose Bengal
Average Rating: 5 (Based on 1 Review.)
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works well.
By
Anonymous on 03/29/2020
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: rat adrenal medulla chromaffin cells
100 μM
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals