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Cat.No. 6649 - RADA | C28H28N4O6
Description: Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Alternative Names: TADA
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)

Biological Activity

Orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.

This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content,

RADA Applications

Application of RADA

Using FDAAs RADA and HADA (Cat. No 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm.

For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841

Optical Data for RADA

RADA Dye Spectra
Emission Color Orange
λabs 554 nm
λem 580 nm
Extinction Coefficient (ε) 95000 M-1cm-1
Quantum Yield (φ) 0.69

Technical Data

M. Wt 516.55
Formula C28H28N4O6
Storage Store at -20°C
Purity ≥98% (HPLC)
Smiles N[C@@H](C(O)=O)CNC(C1=CC=CC(C(C2=C(C=C(N(C)C)C=C2)O3)=C4C3=C/C(C=C4)=[N+](C)/C)=C1C([O-])=O)=O.N[C@@H](C(O)=O)CNC(C5=CC(C([O-])=O)=C(C(C6=C(C=C(N(C)C)C=C6)O7)=C8C7=C/C(C=C8)=[N+](C)/C)C=C5)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 51.66 100

Preparing Stock Solutions

The following data is based on the product molecular weight 516.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.94 mL 9.68 mL 19.36 mL
5 mM 0.39 mL 1.94 mL 3.87 mL
10 mM 0.19 mL 0.97 mL 1.94 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665

García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 7 pii: e37243. PMID: 30198841

Fenton et al (2017) Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol. 2 17011 PMID: 28112724

If you know of a relevant reference for RADA, please let us know.

Keywords: RADA, RADA supplier, Fluorescent, D-amino, acid, FFDA, TADA, TAMRA, labeling, peptidoglycans, bacteria, dSTORM, reSTORM, SRM, super, resolution, microscopy, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6649, Tocris Bioscience

2 Citations for RADA

Citations are publications that use Tocris products. Selected citations for RADA include:

Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841

Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906

Do you know of a great paper that uses RADA from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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