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Orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.
This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content,
Optical Data for RADA
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 516.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.94 mL||9.68 mL||19.36 mL|
|5 mM||0.39 mL||1.94 mL||3.87 mL|
|10 mM||0.19 mL||0.97 mL||1.94 mL|
|50 mM||0.04 mL||0.19 mL||0.39 mL|
References are publications that support the biological activity of the product.
Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665
García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 7 pii: e37243. PMID: 30198841
Fenton et al (2017) Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol. 2 17011 PMID: 28112724
If you know of a relevant reference for RADA, please let us know.
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