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Submit ReviewBiotin BromoCatch™ Ligand New
Description: A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
Chemical Name: N-((17R)-17-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-16-oxo-3,6,9,12-tetraoxa-15-azanonadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Purity: ≥95% (HPLC)
Biological Activity for Biotin BromoCatch™ Ligand
Biotin BromoCatch™ Ligand is a covalent probe designed for the selective labeling and enrichment of proteins tagged with the BromoCatch™ self-labeling tag. Based on a para-acrylamide-functionalized bump-JQ1 scaffold, the BromoCatch™ ligand, enables irreversible and highly specific covalent attachment to the engineered cysteine residue in the BromoCatch-tagged protein. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. This probe demonstrates exceptional target selectivity, confirmed via intact protein mass spectrometry and western blotting, and shows no background labeling in untransfected or wild-type samples even at micromolar concentrations. Biotin BromoCatch™ Ligand expands the utility of the BromoCatch™ platform for biochemical workflows including proteomics, immunoprecipitation, and interaction studies.
Please refer to this protocol for further information on how to use this product.
BromoCatch is a trademark of Bio-Techne Corp.
Licensing Information
Sold under exclusive licence from the University of Dundee
Technical Data for Biotin BromoCatch™ Ligand
| M. Wt | 908.15 |
| Formula | C44H61N9O8S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | BZQKBFKRTBEEFV-HQANLXDBSA-N |
| Smiles | CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCOCCNC(CCCC[C@H]4[C@](NC(N5)=O)([H])[C@]5([H])CS4)=O)=O)CC)C6=NN=C(C)N26 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Biotin BromoCatch™ Ligand
References for Biotin BromoCatch™ Ligand
References are publications that support the biological activity of the product.
Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv
If you know of a relevant reference for Biotin BromoCatch™ Ligand, please let us know.
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