HADA

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Cat.No. 6647 - HADA | C13H12N2O6.HCl
Description: Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Results in strong peripheral and septal labeling of diverse bacterial cell populations without affecting growth rates. Displays mitochondrial outer membrane permeability. Excitation/emission λ ~405/460 nm.

Technical Data

M. Wt 328.71
Formula C13H12N2O6.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
Smiles N[C@@H](C(O)=O)CNC(C1=CC(C=CC(O)=C2)=C2OC1=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.87 100

Preparing Stock Solutions

The following data is based on the product molecular weight 328.71. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.04 mL 15.21 mL 30.42 mL
5 mM 0.61 mL 3.04 mL 6.08 mL
10 mM 0.3 mL 1.52 mL 3.04 mL
50 mM 0.06 mL 0.3 mL 0.61 mL

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References

References are publications that support the biological activity of the product.

Kuru et al (2012) In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665

Botella et al (2017) Distinct spatiotemporal dynamics of peptidoglycan synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis. Mbio. 8 e01183 PMID: 28900018


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Keywords: HADA, HADA supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, blue, Other, Fluorescent, Probes, Amino, Acids, 6647, Tocris Bioscience

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