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Submit ReviewJanelia Fluor® 635, BromoCatch™ Ligand New
Description: Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Chemical Name: 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Biological Activity
Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.
Please refer to this protocol for further information on how to use this product.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
BromoCatch is a trademark of Bio-Techne Corp.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee
Optical Data for Janelia Fluor® 635, BromoCatch™ Ligand
| λabs | 646 nm |
|---|---|
| λem | 663 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
| M. Wt | 1152.4 |
| Formula | C61H67F2N9O8SSi |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | KUWXUURGPXZQEI-RYOPHROTSA-N |
| Smiles | CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C([Si](C)(C7=CC(N8CC(C8)F)=CC=C57)C)=C/C(C=C6)=[N+]9CC(C/9)F)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.52 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 1152.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.68 mL | 43.39 mL | 86.78 mL |
| 0.5 mM | 1.74 mL | 8.68 mL | 17.36 mL |
| 1 mM | 0.87 mL | 4.34 mL | 8.68 mL |
| 5 mM | 0.17 mL | 0.87 mL | 1.74 mL |
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References
References are publications that support the biological activity of the product.
Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. www.biorxiv.org/content/10.1101/2025.04.07.647551v1
If you know of a relevant reference for Janelia Fluor® 635, BromoCatch™ Ligand, please let us know.
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