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Submit ReviewBromoCatch™ Control Ligand New
Description: Control ligand for use with BromoCatch™
Chemical Name: Methyl (R)-2-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)butanoate
Purity: ≥97% (HPLC)
Biological Activity for BromoCatch™ Control Ligand
BromoCatch™ Control Ligand is a control ligand for use with BromoCatch™. It is a non-covalent ligand that competes for BromoCatch™ probe binding site.
Please refer to this protocol for further information on how to use this product.
BromoCatch is a trademark of Bio-Techne Corp.
Technical Data for BromoCatch™ Control Ligand
| M. Wt | 442.96 |
| Formula | C22H23ClN4O2S |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 2421153-75-3 |
| PubChem ID | 146037626 |
| InChI Key | WLKVOOMXHSBIOW-APWZRJJASA-N |
| Smiles | CC1=C(C)C(C(C2=CC=C(C=C2)Cl)=N[C@@H]([C@H](C(OC)=O)CC)C3=NN=C(C)N43)=C4S1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BromoCatch™ Control Ligand
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 44.3 | 100 | |
| ethanol | 44.3 | 100 |
Preparing Stock Solutions for BromoCatch™ Control Ligand
The following data is based on the product molecular weight 442.96. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.26 mL | 11.29 mL | 22.58 mL |
| 5 mM | 0.45 mL | 2.26 mL | 4.52 mL |
| 10 mM | 0.23 mL | 1.13 mL | 2.26 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.45 mL |
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Product Datasheets for BromoCatch™ Control Ligand
References for BromoCatch™ Control Ligand
References are publications that support the biological activity of the product.
Runcie et al (2018) Optimization of a "bump-and-hole" approach to allele-selective BET bromodomain inhibition. Chem.Sci. 9 2452 PMID: 29732121
Baud et al (2014) A bump-and-hole approach to engineer controlled selectivity of BET bromodomain chemical probes. Science 346 638 PMID: 25323695
Rodriguez-Rios et al (2025) BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. www.biorxiv.org/content/10.1101/2025.04.07.647551v1
If you know of a relevant reference for BromoCatch™ Control Ligand, please let us know.
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Citations for BromoCatch™ Control Ligand
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