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Red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.
Optical Data for Propidium iodide
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||3.34||5 with sonication|
Preparing Stock Solutions
The following data is based on the product molecular weight 668.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.05 mM||29.92 mL||149.61 mL||299.23 mL|
|0.25 mM||5.98 mL||29.92 mL||59.85 mL|
|0.5 mM||2.99 mL||14.96 mL||29.92 mL|
|2.5 mM||0.6 mL||2.99 mL||5.98 mL|
References are publications that support the biological activity of the product.
Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766
Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001
Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180
If you know of a relevant reference for Propidium iodide, please let us know.
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Keywords: Propidium iodide, Propidium iodide supplier, red, fluorescent, DNA, stain, dye, cell, death, PI, Fluorescent, Stains, Cell, Viability, and, Dyes, Flow, Cytometry, 5135, Tocris Bioscience
Citations for Propidium iodide
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Reviews for Propidium iodide
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PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.
Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.
5mM is great for visualization with an mcherry filter. It goes into water well.
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