Propidium iodide

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Cat.No. 5135 - Propidium iodide | C27H34I2N4 | CAS No. 25535-16-4
Description: Red-fluorescent DNA stain; membrane impermeant to live cells
Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Purity: ≥95% (HPLC)

Biological Activity

Red-fluorescent DNA stain. Membrane impermeant to live cells; differentiates between live and dead cells in cell populations.

Technical Data

M. Wt 668.39
Formula C27H34I2N4
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 25535-16-4
PubChem ID 104981
Smiles NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 3.34 5mM with sonication

Preparing Stock Solutions

The following data is based on the product molecular weight 668.39. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.5 mL 7.48 mL 14.96 mL
5 mM 0.3 mL 1.5 mL 2.99 mL
10 mM 0.15 mL 0.75 mL 1.5 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766

Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001

Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180

If you know of a relevant reference for Propidium iodide, please let us know.

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Literature in this Area

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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

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