Propidium iodide

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Description: Red-fluorescent DNA stain; membrane impermeant to live cells. PI staining differentiates live and dead cells
Alternative Names: PI
Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Purity: ≥95% (HPLC)
Reviews (2)
Literature (1)

Biological Activity for Propidium iodide

Propidium iodide is a red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.

Optical Data for Propidium iodide

Propidium iodide Dye Spectra
Emission Color Orange
λabs 535 nm
λem 617 nm
Application Flow Cytometry, Fluorescence Microscopy, Confocal Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Propidium iodide

M. Wt 668.39
Formula C27H34I2N4
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 25535-16-4
PubChem ID 104981
Smiles NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Propidium iodide

Solvent Max Conc. mg/mL Max Conc. mM
water 3.34 5 with sonication

Preparing Stock Solutions for Propidium iodide

The following data is based on the product molecular weight 668.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 29.92 mL 149.61 mL 299.23 mL
0.25 mM 5.98 mL 29.92 mL 59.85 mL
0.5 mM 2.99 mL 14.96 mL 29.92 mL
2.5 mM 0.6 mL 2.99 mL 5.98 mL

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Product Datasheets for Propidium iodide

Certificate of Analysis / Product Datasheet
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References for Propidium iodide

References are publications that support the biological activity of the product.

Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766

Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001

Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180

If you know of a relevant reference for Propidium iodide, please let us know.

Keywords: Propidium iodide, Propidium iodide supplier, red, fluorescent, DNA, stain, dye, cell, death, PI, Fluorescent, Stains, Cell, Viability, and, Dyes, Flow, Cytometry, 5135, Tocris Bioscience

Citations for Propidium iodide

Citations are publications that use Tocris products.

Currently there are no citations for Propidium iodide. Do you know of a great paper that uses Propidium iodide from Tocris? Please let us know.

Reviews for Propidium iodide

Average Rating: 4.5 (Based on 2 Reviews.)

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Good Product.
By Rohit Sharma on 08/17/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Jurkat Cells

PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.

review image

Propidium dye for intracellular fluorescent dye..
By Anonymous on 03/13/2018
Assay Type: Ex Vivo
Species: Mouse

Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.

5mM is great for visualization with an mcherry filter. It goes into water well.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

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