Propidium iodide

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Cat.No. 5135 - Propidium iodide | C27H34I2N4 | CAS No. 25535-16-4
Description: Red-fluorescent DNA stain; membrane impermeant to live cells
Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Purity: ≥95% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Red-fluorescent DNA stain. Membrane impermeant to live cells; differentiates between live and dead cells in cell populations.

Technical Data

M. Wt 668.39
Formula C27H34I2N4
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 25535-16-4
PubChem ID 104981
InChI Key XJMOSONTPMZWPB-UHFFFAOYSA-M
Smiles NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 3.34 5mM with sonication

Preparing Stock Solutions

The following data is based on the product molecular weight 668.39. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.5 mL 7.48 mL 14.96 mL
5 mM 0.3 mL 1.5 mL 2.99 mL
10 mM 0.15 mL 0.75 mL 1.5 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766

Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001

Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180


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Citations for Propidium iodide

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Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for Propidium iodide

Protocols

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