Propidium iodide
Biological Activity
Red-fluorescent DNA stain. Membrane impermeant to live cells; differentiates between live and dead cells in cell populations.
Technical Data
| M. Wt | 668.39 |
| Formula | C27H34I2N4 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 25535-16-4 |
| PubChem ID | 104981 |
| InChI Key | XJMOSONTPMZWPB-UHFFFAOYSA-M |
| Smiles | NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.34 | 5 with sonication |
Preparing Stock Solutions
The following data is based on the product molecular weight 668.39. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 29.92 mL | 149.61 mL | 299.23 mL |
| 0.25 mM | 5.98 mL | 29.92 mL | 59.85 mL |
| 0.5 mM | 2.99 mL | 14.96 mL | 29.92 mL |
| 2.5 mM | 0.6 mL | 2.99 mL | 5.98 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the biological activity of the product.
Jeong et al (2013) SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 289 4135 PMID: 24368766
Sonnemann et al (2014) p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 110 656 PMID: 24281001
Yun et al (2013) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 289 1491 PMID: 24297180
If you know of a relevant reference for Propidium iodide, please let us know.
View Related Products by Target
Keywords: Propidium iodide, Propidium iodide supplier, red, fluorescent, DNA, stain, dye, cell, death, Fluorescent, Stains, 5135, Tocris Bioscience
Citations for Propidium iodide
Citations are publications that use Tocris products.
Currently there are no citations for Propidium iodide. Do you know of a great paper that uses Propidium iodide from Tocris? Please let us know.
Reviews for Propidium iodide
Average Rating: 4 (Based on 1 Review.)
Have you used Propidium iodide?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.
5mM is great for visualization with an mcherry filter. It goes into water well.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Fall/Winter 2018/2019)New
Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.
- 7-TM Receptors
- Enzymes
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Transporters
- Chemogenetics
- Epigenetics
- Fluorescent Imaging
- PROTACs
- Signal Transduction
- Stem Cells
