Pricing Availability   Qty
Description: preQ1 with azide handle for click chemistry
Chemical Name: N-[6-[[(2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]amino]hexyl]-3-(2-azidoethoxy)propanamide
Purity: ≥95% (HPLC)

Biological Activity for preQ1-azide

preQ1-azide is a modified analog of the natural substrate prequeosine1 (preQ1). It contains an azide handle allowing attachment of additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. preQ1-azide is a toolbox compound for RNA-TAG (transglycosylation at guanosine).

Other RNA-TAG toolbox compounds include preQ1-biotin (Cat. No. 7804) and preQ1- alkyne (Cat. No. 7806)

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

Licensing Information

Sold under license from The Regents of the University of California.

Technical Data for preQ1-azide

M. Wt 419.49
Formula C18H29N9O3
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for preQ1-azide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 41.95 100
ethanol 20.97 50

Preparing Stock Solutions for preQ1-azide

The following data is based on the product molecular weight 419.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.38 mL 11.92 mL 23.84 mL
5 mM 0.48 mL 2.38 mL 4.77 mL
10 mM 0.24 mL 1.19 mL 2.38 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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References for preQ1-azide

References are publications that support the biological activity of the product.

Zhang et al (2022) Site-specific and enzymatic cross-linking of sgRNA enables wavelength-selectable photoactivated control of CRISPR gene editing. J.Am.Chem.Soc. 144 4487 PMID: 35257575

If you know of a relevant reference for preQ1-azide, please let us know.

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Keywords: preQ1-azide, preQ1-azide supplier, preQ1, azide, handle, click, chemistry, photoactivatable, probes, RNA, TAG, RNATAG, CuAAC, copper-catalyzed, catalysed, Cu(I), Biochemicals, and, Molecular, Biology, 7833, Tocris Bioscience

Citations for preQ1-azide

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