Ponceau S Staining Solution

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Cat.No. 5225 - Ponceau S Staining Solution | C22H12N4Na4O13S4 | CAS No. 6226-79-5
Description: Stain used to detect protein bands after Western blotting
Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
Reviews (3)
Literature (1)

Biological Activity

Stain used to detect protein bands after Western blotting.

Technical Data

M. Wt 760.57
Formula C22H12N4Na4O13S4
Storage Store at RT
CAS Number 6226-79-5
PubChem ID 101933731
Smiles OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solubility Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)

Product Datasheets

Certificate of Analysis / Product Datasheet
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Reviews for Ponceau S Staining Solution

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Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation..
By Anonymous on 12/23/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).

The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.

review image

Best staining for tracking protein transfer in western blot.
By Luiz Alexandre Magno on 07/03/2018
Assay Type: In Vitro
Species: Mouse

We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.

1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections

PMID: 26585834
review image

Western blot confirmation.
By Anonymous on 01/05/2018
Assay Type: In Vitro
Species: Human

Western blot confirmation

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