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Ponceau S Staining Solution
Stain used to detect protein bands after Western blotting.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)|
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Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).
The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.
We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.
1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections
Western blot confirmation
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