Ponceau S Staining Solution

Pricing Availability   Qty
Cat.No. 5225 - Ponceau S Staining Solution | C22H12N4Na4O13S4 | CAS No. 6226-79-5
Description: Stain used to detect protein bands after Western blotting
Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
Datasheet
Citations
Reviews (2)
Literature

Biological Activity

Stain used to detect protein bands after Western blotting.

Technical Data

M. Wt 760.57
Formula C22H12N4Na4O13S4
Storage Store at RT
CAS Number 6226-79-5
PubChem ID 101933731
InChI Key KQHKSGRIBYJYFX-UHFFFAOYSA-J
Smiles OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solubility Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:

References

References are publications that support the biological activity of the product.

If you know of a relevant reference for Ponceau S Staining Solution, please let us know.

View Related Products by Target

Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience

Citations for Ponceau S Staining Solution

Citations are publications that use Tocris products.

Currently there are no citations for Ponceau S Staining Solution. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? Please let us know.

Reviews for Ponceau S Staining Solution

Average Rating: 4.5 (Based on 2 Reviews.)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Ponceau S Staining Solution?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Best staining for tracking protein transfer in western blot
By Luiz Alexandre Magno on 07/03/2018
Assay Type: In Vitro
Species: Mouse

We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.

1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections

PMID: 26585834
review image

Western blot confirmation
By Anonymous on 01/05/2018
Assay Type: In Vitro
Species: Human

Western blot confirmation


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


New Product Guide

New Product Guide (Spring/Summer 2018)

Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Aptamers
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells