Ponceau S Staining Solution
Stain used to detect protein bands after Western blotting.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)|
References are publications that support the biological activity of the product.
If you know of a relevant reference for Ponceau S Staining Solution, please let us know.
View Related Products by Target
Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience
Citations for Ponceau S Staining Solution
Citations are publications that use Tocris products.
Currently there are no citations for Ponceau S Staining Solution. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? Please let us know.
Reviews for Ponceau S Staining Solution
Average Rating: 4.5 (Based on 2 Reviews.)
Have you used Ponceau S Staining Solution?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.
1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections
Western blot confirmation
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Fall/Winter 2018/2019)New
Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells