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Description: TLR3 agonist
Chemical Name: Polyinosinic-polycytidylic acid sodium salt
Citations (20)
Reviews (1)
Pathways (1)

Biological Activity for Poly(I:C)

Poly(I:C) is a synthetic double-stranded RNA (dsRNA); endosomal Toll-like receptor 3 (TLR3) agonist. It also binds to the cytoplasmic receptors, retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA-5), elicits the secretion of type I interferon (IFN) and pro-inflammatory cytokines. Poly(I:C) is commonly used for immune system activation in vivo. Poly(I:C) also promotes maturation of hPSC-derived cardiomyocytes by modulating early Notch signaling and cardiomyogenic transcriptional programs. Poly(I:C) promotes generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075 (Cat. No. 6142). Transfection into NIT-1 β cells induces β-cell apoptosis.

To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations.

Technical Data for Poly(I:C)

Formula (C10H13N4O8P)x.(C9H14N3O8P)x
Storage Store at -20°C
CAS Number 42424-50-0
PubChem ID 32744

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Poly(I:C)

Solubility Soluble to 3 mg/ml in water

Product Datasheets for Poly(I:C)

Certificate of Analysis / Product Datasheet
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References for Poly(I:C)

References are publications that support the biological activity of the product.

Robbins et al (2003) Nuclear factor-κB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor-associated ligand (TRAIL). Endocrinology 144 4616 PMID: 12960048

Rouas et al (2004) Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int.Immunol. 16 767 PMID: 15096480

West et al (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472 476 PMID: 21525932

Spranger et al (2010) Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J.Immunol. 185 738 PMID: 20511554

Biermann et al (2019) Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation. Stem Cells 37 910 PMID: 31087611

Waele et al (2021) A systematic review on poly(I:C) and poly-ICLC in glioblastoma: adjuvants coordinating the unlocking of immunotherapy. J.Exp.Clin.Cancer Res. 40 213 PMID: 34172082

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Keywords: Poly(I:C), Poly(I:C) supplier, Poly(I:C), immunostimulant, toll-like, receptor, TLR, TLR3, agonist, inosinic, acid, cytidylic, double-stranded, RNA, dsRNA, synthetic, maternal, immune, activation, MIA, cardiomyocyte, differentiation, maturation, RIG-I, MDA-5, IFN, Notch, signaling, Toll-like, Receptors, Cardiomyocyte, Stem, Cells, 4287, Tocris Bioscience

20 Citations for Poly(I:C)

Citations are publications that use Tocris products. Selected citations for Poly(I:C) include:

Shahram et al (2021) EZH2 Inhibition Compromises α4-1BB-Mediated Antitumor Efficacy by Reducing the Survival and Effector Programming of CD8+ T Cells. Front Immunol 12 770080 PMID: 34925340

Ivan et al (2021) PERK Is Critical for Alphavirus Nonstructural Protein Translation. Viruses 13 PMID: 34065980

Yi et al (2021) ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages. Arterioscler Thromb Vasc Biol 41 1854-1873 PMID: 33882688

Veronica Elizabeth et al (2021) Virus Infection Induces Keap1 Binding to Cytokine Genes, Which Recruits NF-κB p50 and G9a-GLP and Represses Cytokine Transcription. J Immunol 207 1437-1447 PMID: 34400522

Tröscher et al (2019) Microglial nodules provide the environment for pathogenic T cells in human encephalitis. Acta Neuropathol 137 619 PMID: 30663001

Yu et al (2018) Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis. Mol Cell 72 999 PMID: 30449722

Scott W et al (2022) TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Cell Stem Cell 29 298-314.e9 PMID: 35045331

Xuefei et al (2022) Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions. BMC Biol 20 14 PMID: 35027054

Takashi et al (2022) Human Lung Fibroblasts Exhibit Induced Inflammation Memory via Increased IL6 Gene Expression and Release. Front Immunol 13 921728 PMID: 35941890

Sandra et al (2022) Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay. STAR Protoc 3 101866 PMID: 36595902

Johann et al (2022) Pro-inflammatory activation changes intracellular transport of bevacizumab in the retinal pigment epithelium in vitro. Graefes Arch Clin Exp Ophthalmol 260 857-872 PMID: 34643794

Nicolas et al (2019) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence. Brain Behav Immun 79 274-283 PMID: 30776474

Tachibana et al (2022) Poly I:C and R848 facilitate nitric oxide production via inducible nitric oxide synthase in chicks. Comp.Biochem.Physiol.A Mol.Integr.Physiol. 269 111211 PMID: 35417747

Mingjun et al (2023) A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3. iScience 26 105935 PMID: 36685041

Alyssa H et al (2023) Weight cycling induces innate immune memory in adipose tissue macrophages. Front Immunol 13 984859 PMID: 36713396

Anne et al (2023) Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe. Cell Genom 3 100248 PMID: 36819665

Alexa et al (2023) Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells. Heliyon 9 e15202 PMID: 37123974

Hideaki et al (2020) TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis. Front Immunol 11 98 PMID: 32117252

Masayuki et al (2016) Systemic Activation of TLR3-Dependent TRIF Signaling Confers Host Defense against Gram-Negative Bacteria in the Intestine. Front Cell Infect Microbiol 5 105 PMID: 26793623

Yue et al (2018) PDGFRβ Cells Rapidly Relay Inflammatory Signal from the Circulatory System to Neurons via Chemokine CCL2. Neuron 100 183-200.e8 PMID: 30269986

Do you know of a great paper that uses Poly(I:C) from Tocris? Please let us know.

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Poly IC.
By shubham dubey on 12/22/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: 16HBE

To activate TLR3

review image

Pathways for Poly(I:C)

Toll-like Receptor Signaling Pathway

Toll-like Receptor Signaling Pathway

TLR signaling is involved in the cellular response to threatening molecules such as bacteria and viruses. It results in an inflammatory and immmunological response.