PNU 74654

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Description: β-catenin binder; inhibits Wnt signaling
Chemical Name: 2-Phenoxybenzoic acid-[(5-methyl-2-furanyl)methylene]hydrazide
Purity: ≥99% (HPLC)
Datasheet
Citations (9)
Reviews (1)
Pathways (1)

Biological Activity for PNU 74654

Binds to β-catenin (KD = 450 nM). Inhibits the interaction between β-catenin and T cell factor 4 (Tcf4) and disrupts the Wnt signaling pathway. Promotes neural differentiation of hPSCs as part of a chemical cocktail.

Compound Libraries for PNU 74654

PNU 74654 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for PNU 74654

M. Wt 320.34
Formula C19H16N2O3
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 113906-27-7
PubChem ID 9836739
InChI Key JJEDWBQZCRESJL-DEDYPNTBSA-N
Smiles O=C(N/N=C/C2=CC=C(C)O2)C1=C(OC3=CC=CC=C3)C=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PNU 74654

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.03 100

Preparing Stock Solutions for PNU 74654

The following data is based on the product molecular weight 320.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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References for PNU 74654

References are publications that support the biological activity of the product.

Trosset et al (2006) Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening. Proteins 64 60 PMID: 16568448

Wells and McClendon (2007) Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature 450 1001 PMID: 18075579

Chen et al (2019) Chemically defined neural conversion of human pluripotent stem cells. Methods Mol.Biol. 1919 59 PMID: 30656621


If you know of a relevant reference for PNU 74654, please let us know.

Keywords: PNU 74654, PNU 74654 supplier, PNU74654, beta-catenin, b-catenin, β-catenin, binders, wnt, signalling, signaling, neural, stem, cell, differentiation, Beta-catenin, Neural, Stem, Cells, 3534, Tocris Bioscience

9 Citations for PNU 74654

Citations are publications that use Tocris products. Selected citations for PNU 74654 include:

Juan C et al (2021) α-Synuclein Oligomers Induce Glutamate Release from Astrocytes and Excessive Extrasynaptic NMDAR Activity in Neurons, Thus Contributing to Synapse Loss. J Neurosci 41 2264-2273 PMID: 33483428

Stephen J et al (2021) Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior. Genes Dev 35 1527-1547 PMID: 34711655

Chen et al (2019) Chemically defined neural conversion of human pluripotent stem cells. Methods Mol.Biol. 1919 59 PMID: 30656621

Margaret E et al (2023) FSCN1 as a new druggable target in adrenocortical carcinoma. Int J Cancer 153 210-223 PMID: 36971100


Do you know of a great paper that uses PNU 74654 from Tocris? Please let us know.

Reviews for PNU 74654

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Neural Induction Conditions.
By Anonymous on 03/22/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: H9

2 μM PNU-74654

PMID: 30656621
review image

Pathways for PNU 74654

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.