PNU 74654

Pricing Availability   Qty
Description: β-catenin binder; inhibits Wnt signaling
Chemical Name: 2-Phenoxybenzoic acid-[(5-methyl-2-furanyl)methylene]hydrazide
Purity: ≥99% (HPLC)
Citations (9)
Reviews (1)
Literature (1)
Pathways (1)

Biological Activity for PNU 74654

Binds to β-catenin (KD = 450 nM). Inhibits the interaction between β-catenin and T cell factor 4 (Tcf4) and disrupts the Wnt signaling pathway. Promotes neural differentiation of hPSCs as part of a chemical cocktail.

Compound Libraries for PNU 74654

PNU 74654 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for PNU 74654

M. Wt 320.34
Formula C19H16N2O3
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 113906-27-7
PubChem ID 9836739
Smiles O=C(N/N=C/C2=CC=C(C)O2)C1=C(OC3=CC=CC=C3)C=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PNU 74654

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 32.03 100

Preparing Stock Solutions for PNU 74654

The following data is based on the product molecular weight 320.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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References for PNU 74654

References are publications that support the biological activity of the product.

Trosset et al (2006) Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening. Proteins 64 60 PMID: 16568448

Wells and McClendon (2007) Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature 450 1001 PMID: 18075579

Chen et al (2019) Chemically defined neural conversion of human pluripotent stem cells. Methods Mol.Biol. 1919 59 PMID: 30656621

If you know of a relevant reference for PNU 74654, please let us know.

Keywords: PNU 74654, PNU 74654 supplier, PNU74654, beta-catenin, b-catenin, β-catenin, binders, wnt, signalling, signaling, neural, stem, cell, differentiation, Beta-catenin, Neural, Stem, Cells, 3534, Tocris Bioscience

9 Citations for PNU 74654

Citations are publications that use Tocris products. Selected citations for PNU 74654 include:

Corritore et al (2014) β-Cell differentiation of human pancreatic duct-derived cells after in vitro expansion. Ann Neurol 16 456 PMID: 25437872

Baril et al (2013) Genome-wide RNAi screen reveals a new role of a WNT/CTNNB1 signaling pathway as negative regulator of virus-induced innate immune responses. PLoS Pathog 9 e1003416 PMID: 23785285

Liao et al (2015) Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Nature 47 469 PMID: 25822089

Tsankov et al (2015) Transcription factor binding dynamics during human ES cell differentiation. Stem Cells Int 518 344 PMID: 25693565

Do you know of a great paper that uses PNU 74654 from Tocris? Please let us know.

Reviews for PNU 74654

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Neural Induction Conditions.
By Anonymous on 03/22/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: H9

2 μM PNU-74654

PMID: 30656621
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for PNU 74654

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.