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Pimonidazole is a hypoxia detection reagent. Labels hypoxic tumor cells in vivo. Also labels hypoxic mesenchymal stem cells in mouse bone marrow.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 254.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.93 mL||19.66 mL||39.33 mL|
|5 mM||0.79 mL||3.93 mL||7.87 mL|
|10 mM||0.39 mL||1.97 mL||3.93 mL|
|50 mM||0.08 mL||0.39 mL||0.79 mL|
References are publications that support the biological activity of the product.
Sato et al (2016) Notch2 signaling regulates the proliferation of murine bone marrow-derived mesenchymal stem/stromal cells via c-Myc expression. PLoS One 11 e0165946 PMID: 27855169
Bennewith et al (2002) Orally administered pimonidazole to label hypoxic tumor cells. Cancer Res. 62 6827 PMID: 12460894
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Keywords: Pimonidazole, Pimonidazole supplier, hypoxia, detection, detects, hypoxic, in, vivo, labels, labeling, Labels, and, Stains, Fluorescent, Cell, Indicators, Sensors, 6182, Tocris Bioscience
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Glioblstoma patient derived cell line E2 was cultured in a 3D model for 21 days and the level of hypoxia in the spheres were measured following treatment with Pimonidazole.
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