Phen Green SK diacetate

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Description: Heavy metal ion indicator, membrane permeable
Chemical Name: 3',6'-Bis(acetyloxy)-2',7'-dichloro-3-oxo-N-1,10-phenanthrolin-5-yl-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxamide
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for Phen Green SK diacetate

Phen Green SK diacetate is a fluorescent heavy metal ion indicator that can be used to detect various metal ions including Cu2+, Cu+, Fe2+, Hg2+, Pb2+, Cd2+, Zn2+ and Ni2+. Excitation and emission maxima (λ) are 507 and 532 nm, respectively. Intracellular Phen Green SK diacetate fluorescence is quenched upon reaction with metal ions. This product is cell permeable.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Phen Green SK diacetate

λabs 507 nm
λem 532 nm
Extinction Coefficient (ε) 40000 M-1cm-1
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry

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Technical Data for Phen Green SK diacetate

M. Wt 706.49
Formula C37H21Cl2N3O8
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 234075-45-7
PubChem ID 137700453
Smiles O=C1OC2(C3=CC=C(C(NC4=CC5=CC=CN=C5C6=C4C=CC=N6)=O)C=C13)C7=CC(Cl)=C(C=C7OC8=CC(OC(C)=O)=C(C=C82)Cl)OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Phen Green SK diacetate

References for Phen Green SK diacetate

References are publications that support the biological activity of the product.

Shen et al (2022) Geniposide possesses the protective effect on myocardial injury by inhibiting oxidative stress and ferroptosis via activation of the Grsf1/GPx4 Axis. Front.Pharmacol. 13 879870 PMID: 35600863

Kim et al (2006) Calibration of Phen Green for use as a Cu(I)-selective fluorescent indicator. Anal.Chim.Acta 575 223 PMID: 17723595

Lu et al (2022) Epigenetic regulation of ferroptosis via ETS1/miR-23a-3p/ACSL4 axis mediates sorafenib resistance in human hepatocellular carcinoma. J.Exp.Clin.Cancer Res. 41 3 PMID: 34980204

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Citations for Phen Green SK diacetate

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