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Submit ReviewPhalloidin-Janelia Fluor® 646
Description: Red fluorescent F-actin probe
Alternative Names: Phalloidin-JF646
Purity: ≥90% (HPLC)
Biological Activity for Phalloidin-Janelia Fluor® 646
Phalloidin-Janelia Fluor® 646 is a red fluorescent F-actin probe. Phalloidin-Janelia Fluor® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor® 646 (Cat. No. 6148). It is suitable for use in confocal and super-resolution microscopy techniques such as dSTORM. Excitation/emission maximum λ = 646/664 nm.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Phalloidin-Janelia Fluor® 646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Application | Confocal imaging, dSTORM, STED, Flow Cytometry |
Technical Data for Phalloidin-Janelia Fluor® 646
| M. Wt | 1266.51 |
| Formula | C64H75N11O13SSi |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | AOXMEZGPCIYGOB-BNAQDRCBSA-N |
| Smiles | O=C([C@H](NC([C@H](C)NC([C@H](C[C@](O)(C)CNC(C1=CC=C(C([O-])=O)C(C2=C(C=C/3)C([Si](C)(C)C4=CC(N5CCC5)=CC=C42)=CC3=[N+]6CCC\6)=C1)=O)N7)=O)=O)[C@H](C)O)N[C@H]8CSC(N9)=C(C[C@H](NC([C@H](C)NC([C@@H]%10C[C@@H](O)CN%10C8=O)=O)=O)C7=O)C%11=C9C=CC=C%11 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phalloidin-Janelia Fluor® 646
References for Phalloidin-Janelia Fluor® 646
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
Cooper et al (1987) Effects of cytochalasin and phalloidin on actin. J.Cell.Biol. 105 1473 PMID: 3312229
If you know of a relevant reference for Phalloidin-Janelia Fluor® 646, please let us know.
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Citations for Phalloidin-Janelia Fluor® 646
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