Phalloidin-Janelia Fluor® 646

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Description: Red fluorescent F-actin probe
Alternative Names: Phalloidin-JF646
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for Phalloidin-Janelia Fluor® 646

Phalloidin-Janelia Fluor® 646 is a red fluorescent F-actin probe. Phalloidin-Janelia Fluor® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor® 646 (Cat. No. 6148). It is suitable for use in confocal and super-resolution microscopy techniques such as dSTORM. Excitation/emission maximum λ = 646/664 nm.

Scientific Data

Confocal Microscopy Application of Phalloidin-Janelia Fluor® 646 View Larger

Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.

Confocal Microscopy Application of Phalloidin-Janelia Fluor® 646 View Larger

Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Phalloidin-Janelia Fluor® 646

Phalloidin-Janelia Fluor<sup>®</sup> 646 Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Application Confocal Imaging, dSTORM, STED, Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Phalloidin-Janelia Fluor® 646

M. Wt 1266.51
Formula C64H75N11O13SSi
Storage Store at -20°C
Purity ≥90% (HPLC)
InChI Key AOXMEZGPCIYGOB-BNAQDRCBSA-N
Smiles O=C([C@H](NC([C@H](C)NC([C@H](C[C@](O)(C)CNC(C1=CC=C(C([O-])=O)C(C2=C(C=C/3)C([Si](C)(C)C4=CC(N5CCC5)=CC=C42)=CC3=[N+]6CCC\6)=C1)=O)N7)=O)=O)[C@H](C)O)N[C@H]8CSC(N9)=C(C[C@H](NC([C@H](C)NC([C@@H]%10C[C@@H](O)CN%10C8=O)=O)=O)C7=O)C%11=C9C=CC=C%11

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Phalloidin-Janelia Fluor® 646

References for Phalloidin-Janelia Fluor® 646

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Cooper et al (1987) Effects of cytochalasin and phalloidin on actin. J.Cell.Biol. 105 1473 PMID: 3312229


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Keywords: Phalloidin-Janelia Fluor 646, Phalloidin-Janelia Fluor 646 supplier, Phalloidin, Janelia, Fluor, 646, red, fluorescent, F-actin, probes, dyes, stains, cytoskeletal, cytoskeleton, Phalloidin-JF646, Fluorescent, Actin, Probes, 7201, Tocris Bioscience

Citations for Phalloidin-Janelia Fluor® 646

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