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Description: Puromycin analog for labeling newly synthesized proteins
Chemical Name: 3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine
Purity: ≥97% (HPLC)

Biological Activity for O-Propargyl-puromycin

O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.

Optical Data for O-Propargyl-puromycin

λem 275 nm
Reactivity Click
Application Fluorescence Microscopy, Flow Cytometry

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Technical Data for O-Propargyl-puromycin

M. Wt 495.54
Formula C24H29N7O5
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 1416561-90-4
PubChem ID 71576433
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for O-Propargyl-puromycin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 49.55 100

Preparing Stock Solutions for O-Propargyl-puromycin

The following data is based on the product molecular weight 495.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.09 mL 20.18 mL
5 mM 0.4 mL 2.02 mL 4.04 mL
10 mM 0.2 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

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Product Datasheets for O-Propargyl-puromycin

References for O-Propargyl-puromycin

References are publications that support the biological activity of the product.

Liu et al (2012) Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 109 413 PMID: 22160674

Morral et al (2020) Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 26 845 PMID: 32396863

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Citations for O-Propargyl-puromycin

Citations are publications that use Tocris products.

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