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O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.
|Application||Fluorescence Microscopy, Flow Cytometry|
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 495.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.02 mL||10.09 mL||20.18 mL|
|5 mM||0.4 mL||2.02 mL||4.04 mL|
|10 mM||0.2 mL||1.01 mL||2.02 mL|
|50 mM||0.04 mL||0.2 mL||0.4 mL|
References are publications that support the biological activity of the product.
Liu et al (2012) Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 109 413 PMID: 22160674
Morral et al (2020) Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 26 845 PMID: 32396863
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