Pricing Availability   Qty
Cat.No. 7391 - O-Propargyl-puromycin | C24H29N7O5 | CAS No. 1416561-90-4
Description: Puromycin analog for labeling newly synthesized proteins; is incorporated into nascent polypeptides
Chemical Name: 3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine
Purity: ≥97% (HPLC)
Literature (1)

Biological Activity for O-Propargyl-puromycin

O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.

Optical Data for O-Propargyl-puromycin

λem 275 nm
Reactivity Click
Application flow cytometry

Technical Data for O-Propargyl-puromycin

M. Wt 495.54
Formula C24H29N7O5
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 1416561-90-4
PubChem ID 71576433
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for O-Propargyl-puromycin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 49.55 100

Preparing Stock Solutions for O-Propargyl-puromycin

The following data is based on the product molecular weight 495.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.09 mL 20.18 mL
5 mM 0.4 mL 2.02 mL 4.04 mL
10 mM 0.2 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for O-Propargyl-puromycin

References for O-Propargyl-puromycin

References are publications that support the biological activity of the product.

Liu et al (2012) Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 109 413 PMID: 22160674

Morral et al (2020) Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 26 845 PMID: 32396863

If you know of a relevant reference for O-Propargyl-puromycin, please let us know.

Keywords: O-Propargyl-puromycin, O-Propargyl-puromycin supplier, imaging, fluorescence, flow, cytometry, puromycin, analog, OP-puro, protein, labelling, probe, labeling, click, conjugation, Other, Fluorescent, Probes, Biochemicals, and, Molecular, Biology, 7391, Tocris Bioscience

Citations for O-Propargyl-puromycin

Citations are publications that use Tocris products.

Currently there are no citations for O-Propargyl-puromycin. Do you know of a great paper that uses O-Propargyl-puromycin from Tocris? Please let us know.

Reviews for O-Propargyl-puromycin

There are currently no reviews for this product. Be the first to review O-Propargyl-puromycin and earn rewards!

Have you used O-Propargyl-puromycin?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes Research Product Guide

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals