NU 7441

Pricing Availability Delivery Time Qty
Cat.No. 3712 - NU 7441 | C25H19NO3S | CAS No. 503468-95-9
Description: Potent and selective DNA-PK inhibitor
Alternative Names: KU 57788
Chemical Name: 8-(4-Dibenzothienyl)-2-(4-morpholinyl)-4H-1-benzopyran-4-one
Purity: ≥99% (HPLC)
Datasheet
Citations (23)
Literature

Biological Activity

Potent and selective DNA-PK inhibitor (IC50 = 14 nM). Selective for DNA-PK over a range of kinases including mTOR, PI 3-K, ATM and ATR. Potentiates the effects of doxorubicin (Cat. No. 2252) and etoposide (Cat. No. 1226) in vitro and etoposide in vivo. Also enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency 2 to 3-fold, and decreases nonhomologous end-joining (NHEJ) frequency ~40%.

Technical Data

M. Wt 413.49
Formula C25H19NO3S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 503468-95-9
PubChem ID 11327430
InChI Key JAMULYFATHSZJM-UHFFFAOYSA-N
Smiles O=C1C3=C(C(C4=CC=CC5=C4SC6=C5C=CC=C6)=CC=C3)OC(N2CCOCC2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2.07 5mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 413.49. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.42 mL 12.09 mL 24.18 mL
5 mM 0.48 mL 2.42 mL 4.84 mL
10 mM 0.24 mL 1.21 mL 2.42 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Zhao et al (2006) Preclinical evaluation of a potent novel DNA-dependent protein kinase inhibitor NU7441. Cancer Res. 66 5354 PMID: 16707462

Willmore et al (2008) DNA-dependent protein kinase is a therapeutic target and an indicator of poor prognosis in B-cell chronic lymphocytic leukemia. Clin.Cancer Res. 14 3984 PMID: 18559621

Leahy et al (2004) Identification of a highly potent and selective DNA-dependent protein kinase (DNA-PK) inhibitor (NU7441) by screening of chromenone libraries. Bioorg.Med.Chem.Lett. 14 6083 PMID: 15546735

Robert et al (2015) Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing. Genome Med. 7 93 PMID: 26307031


If you know of a relevant reference for NU 7441, please let us know.

View Related Products by Product Action

View all DNA-Dependent Protein Kinase Inhibitors

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23 Citations for NU 7441

Citations are publications that use Tocris products. Selected citations for NU 7441 include:

Yamamoto et al (2012) Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice. J Virol 198 305 PMID: 22869596

Bohgaki et al (2011) Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome. PLoS Genet 7 e1001381 PMID: 21552324

Sharma et al (2011) γ-Radiation promotes immunological recognition of cancer cells through increased expression of cancer-testis antigens in vitro and in vivo. Invest Ophthalmol Vis Sci 6 e28217 PMID: 22140550

Cataldi and McCarty (2010) Differential effects of DNA double-strand break repair pathways on single-strand and self-complementary adeno-associated virus vector genomes. Mol Cell Biol 84 8673 PMID: 20538857

Yu et al (2015) NSCLC cells demonstrate differential mode of cell death in response to the combined treatment of radiation and a DNA-PKcs inhibitor. Oncotarget 6 3848 PMID: 25714019

Bunch et al (2015) Transcriptional elongation requires DNA break-induced signalling. Invest Ophthalmol Vis Sci 6 10191 PMID: 26671524

Thakran et al (2015) Pioglitazone restores IGFBP-3 levels through DNA PK in retinal endothelial cells cultured in hyperglycemic conditions. Open Biol 56 177 PMID: 25525174

Colin et al (2015) Cellular responses to a prolonged delay in mitosis are determined by a DNA damage response controlled by Bcl-2 family proteins. Viruses 5 140156 PMID: 25761368

Hollingworth et al (2015) Activation of DNA Damage Response Pathways during Lytic Replication of KSHV. Nucleic Acids Res 7 2908 PMID: 26057167

Rein et al (2015) EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele. Nat Commun 43 7371 PMID: 26160886

Mishra et al (2015) Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase. Mol Cancer Ther 6 6224 PMID: 25692509

Henriksson et al (2014) The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes Dev 28 2726 PMID: 25512560

Zhou et al (2014) Identification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening. BMC Cancer 14 944 PMID: 25495526

Jones et al (2014) BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcitabine. J Cell Biol 13 2412 PMID: 25053826

Zhou and Paull (2013) DNA-dependent protein kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and ataxia telangiectasia-mutated (ATM). J Biol Chem 288 37112 PMID: 24220101

Yüce and West (2013) Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response. Mol Cell Biol 33 406 PMID: 23149945

Edwards et al (2013) Human papillomavirus episome stability is reduced by aphidicolin and controlled by DNA damage response pathways. J Virol 87 3979 PMID: 23365423

Rass et al (2013) Ataxia telangiectasia mutated (ATM) is dispensable for endonuclease I-SceI-induced homologous recombination in mouse embryonic stem cells. J Biol Chem 288 7086 PMID: 23355489

Song et al (2013) Targeting aberrant DNA double-strand break repair in triple-negative breast cancer with alpha-particle emitter radiolabeled anti-EGFR antibody. Mol Cancer Ther 12 2043 PMID: 23873849

Britton et al (2013) A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol 202 579 PMID: 23897892

Oksenych et al (2013) Functional redundancy between the XLF and DNA-PKcs DNA repair factors in V(D)J recombination and nonhomologous DNA end joining. Nat Commun 110 2234 PMID: 23345432

Lee et al (2013) Functional intersection of ATM and DNA-dependent protein kinase catalytic subunit in coding end joining during V(D)J recombination. PLoS One 33 3568 PMID: 23836881

Zhang and Steinle (2013) DNA-PK phosphorylation of IGFBP-3 is required to prevent apoptosis in retinal endothelial cells cultured in high glucose. Neurobiol Dis 54 3052 PMID: 23557743


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Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

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Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

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