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Biological Activity for NR12S
NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cells. In living cells the color emission varies with cholesterol content. Excitation maximum = 554 nm; emission maximum = 627 nm in DMSO.
Optical Data for NR12S
Technical Data for NR12S
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NR12S
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for NR12S
The following data is based on the product molecular weight 695.96. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||14.37 mL||71.84 mL||143.69 mL|
|0.5 mM||2.87 mL||14.37 mL||28.74 mL|
|1 mM||1.44 mL||7.18 mL||14.37 mL|
|5 mM||0.29 mL||1.44 mL||2.87 mL|
References for NR12S
References are publications that support the biological activity of the product.
Kucherak et al (2010) Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J.Am.Chem.Soc. 132 4907 PMID: 20225874
If you know of a relevant reference for NR12S, please let us know.
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Citations for NR12S
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Literature in this Area
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