Pricing Availability   Qty
Cat.No. 7509 - NR12S | C39H57N3O6S | CAS No. 1221739-85-0
Description: Fluorogenic lipid membrane dye
Chemical Name: 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for NR12S

NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cells. In living cells the color emission varies with cholesterol content. Excitation maximum = 554 nm; emission maximum = 627 nm in DMSO.

Optical Data for NR12S

NR12S Dye Spectra
Emission Color Orange
λabs 554 nm
λem 627 nm
Quantum Yield (φ) 0.55
Closest Laser line 532 nm
Application Monitoring of lipid dynamics in living cells
Alternative For Nile Red

Technical Data for NR12S

M. Wt 695.96
Formula C39H57N3O6S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1221739-85-0
PubChem ID 146165932

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NR12S

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 6.96 10

Preparing Stock Solutions for NR12S

The following data is based on the product molecular weight 695.96. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.37 mL 71.84 mL 143.69 mL
0.5 mM 2.87 mL 14.37 mL 28.74 mL
1 mM 1.44 mL 7.18 mL 14.37 mL
5 mM 0.29 mL 1.44 mL 2.87 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for NR12S

References are publications that support the biological activity of the product.

Kucherak et al (2010) Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J.Am.Chem.Soc. 132 4907 PMID: 20225874

If you know of a relevant reference for NR12S, please let us know.

Keywords: NR12S, NR12S supplier, fluorogenic, probe, lipid, cholesterol, membranes, orange, dye, Nile, fluorescence, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7509, Tocris Bioscience

Citations for NR12S

Citations are publications that use Tocris products.

Currently there are no citations for NR12S. Do you know of a great paper that uses NR12S from Tocris? Please let us know.

Reviews for NR12S

There are currently no reviews for this product. Be the first to review NR12S and earn rewards!

Have you used NR12S?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals