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Submit ReviewNile Red
Discontinued Product
7387 has been discontinued.
View all Fluorescent Lipid Probes and Cell Membrane Stains products.
Description: Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy
Alternative Names: Nile blue oxazone
Chemical Name: 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Purity: ≥98% (HPLC)
Biological Activity for Nile Red
Key information: Nile red is a fluorogenic lipid membrane dye.
Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.
Application: fluorescence microscopy.
Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.
Optical Data for Nile Red
| Emission Color | Red |
|---|---|
| λabs | 552 nm |
| λem | 636 nm |
| Application | Fluorescence Microscopy, Monitoring of Lipid Dynamics in Living Cells |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Nile Red
| M. Wt | 318.38 |
| Formula | C20H18N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 7385-67-3 |
| PubChem ID | 65182 |
| InChI Key | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| Smiles | CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Nile Red
References for Nile Red
References are publications that support the biological activity of the product.
Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146
Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737
Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037
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Keywords: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience
Citations for Nile Red
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Currently there are no citations for Nile Red.
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents