Nile Red

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Description: Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy
Alternative Names: Nile blue oxazone
Chemical Name: 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for Nile Red

Key information: Nile red is a fluorogenic lipid membrane dye.

Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.

Application: fluorescence microscopy.

Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.

Optical Data for Nile Red

Nile Red Dye Spectra
Emission Color Red
λabs 552 nm
λem 636 nm
Application Fluorescence Microscopy, Monitoring of Lipid Dynamics in Living Cells

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Technical Data for Nile Red

M. Wt 318.38
Formula C20H18N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 7385-67-3
PubChem ID 65182
Smiles CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Nile Red

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.18 10

Preparing Stock Solutions for Nile Red

The following data is based on the product molecular weight 318.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.41 mL 157.05 mL 314.09 mL
0.5 mM 6.28 mL 31.41 mL 62.82 mL
1 mM 3.14 mL 15.7 mL 31.41 mL
5 mM 0.63 mL 3.14 mL 6.28 mL

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References for Nile Red

References are publications that support the biological activity of the product.

Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146

Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737

Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037

If you know of a relevant reference for Nile Red, please let us know.

Keywords: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience

Citations for Nile Red

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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