Nile Red

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Cat.No. 7387 - Nile Red | C20H18N2O2 | CAS No. 7385-67-3
Description: Fluorogenic lipid membrane dye
Alternative Names: Nile blue oxazone
Chemical Name: 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for Nile Red

Nile red is a fluorogenic lipid membrane dye, which fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Nile red is suitable for long-term monitoring of lipid dynamics in living cells. Excitation maximum = 552 nm; emission maximum = 636 nm in methanol.

Optical Data for Nile Red

Nile Red Dye Spectra
Emission Color Red
λabs 552 nm
λem 636 nm
Application Monitoring of lipid dynamics in living cells

Technical Data for Nile Red

M. Wt 318.38
Formula C20H18N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 7385-67-3
PubChem ID 65182
Smiles CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Nile Red

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.18 10

Preparing Stock Solutions for Nile Red

The following data is based on the product molecular weight 318.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.41 mL 157.05 mL 314.09 mL
0.5 mM 6.28 mL 31.41 mL 62.82 mL
1 mM 3.14 mL 15.7 mL 31.41 mL
5 mM 0.63 mL 3.14 mL 6.28 mL

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References for Nile Red

References are publications that support the biological activity of the product.

Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146

Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737

Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037

If you know of a relevant reference for Nile Red, please let us know.

Keywords: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience

Citations for Nile Red

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