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Nile red is a fluorogenic lipid membrane dye, which fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Nile red is suitable for long-term monitoring of lipid dynamics in living cells. Excitation maximum = 552 nm; emission maximum = 636 nm in methanol.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 318.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||31.41 mL||157.05 mL||314.09 mL|
|0.5 mM||6.28 mL||31.41 mL||62.82 mL|
|1 mM||3.14 mL||15.7 mL||31.41 mL|
|5 mM||0.63 mL||3.14 mL||6.28 mL|
References are publications that support the biological activity of the product.
Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146
Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737
Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037
If you know of a relevant reference for Nile Red, please let us know.
Keywords: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience
Citations are publications that use Tocris products.
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: