MTT

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Cat.No. 5224 - MTT | C18H16N5SBr | CAS No. 298-93-1
Description: Reagent used in the measurement of cell proliferation
Chemical Name: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Purity: ≥98%
Datasheet
Citations
Reviews (2)
Literature

Biological Activity

Reagent used in the measurement of cell proliferation.

Technical Data

M. Wt 414.32
Formula C18H16N5SBr
Storage Store at +4°C
Purity ≥98%
CAS Number 298-93-1
PubChem ID 64965
InChI Key AZKSAVLVSZKNRD-UHFFFAOYSA-M
Smiles CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 5 13

Preparing Stock Solutions

The following data is based on the product molecular weight 414.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.13 mM 18.57 mL 92.83 mL 185.66 mL
0.65 mM 3.71 mL 18.57 mL 37.13 mL
1.3 mM 1.86 mL 9.28 mL 18.57 mL
6.5 mM 0.37 mL 1.86 mL 3.71 mL

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Reconstitution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

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Keywords: MTT, MTT supplier, MTT, measurement, cell, proliferation, Biochemicals, and, Molecular, Biology, 5224, Tocris Bioscience

Citations for MTT

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Reviews for MTT

Average Rating: 4.5 (Based on 2 Reviews.)

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Detection of apoptosis via cell proliferation assay MTT was carried out: Good publishable, would use again.
By Anonymous on 02/11/2019
Species: Human

Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.

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MTT as a tool to estimate cell proliferation: Excellent publishable, performed ideally.
By Anonymous on 12/12/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cardiomyocytes

HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.

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