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Description: Reagent used in the measurement of cell proliferation
Chemical Name: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Purity: ≥98%
Citations (5)
Reviews (2)

Biological Activity for MTT

MTT is a reagent used in the measurement of cell proliferation.

Technical Data for MTT

M. Wt 414.32
Formula C18H16N5SBr
Storage Store at +4°C
Purity ≥98%
CAS Number 298-93-1
PubChem ID 64965
Smiles CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for MTT

Solvent Max Conc. mg/mL Max Conc. mM
PBS 5 13

Preparing Stock Solutions for MTT

The following data is based on the product molecular weight 414.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.13 mM 18.57 mL 92.83 mL 185.66 mL
0.65 mM 3.71 mL 18.57 mL 37.13 mL
1.3 mM 1.86 mL 9.28 mL 18.57 mL
6.5 mM 0.37 mL 1.86 mL 3.71 mL

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Product Datasheets for MTT

Certificate of Analysis / Product Datasheet
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References for MTT

References are publications that support the biological activity of the product.

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Keywords: MTT, MTT supplier, MTT, measurement, cell, proliferation, Biochemicals, and, Molecular, Biology, 5224, Tocris Bioscience

5 Citations for MTT

Citations are publications that use Tocris products. Selected citations for MTT include:

Jeffrey L et al (2023) Aldose reductase inhibition decelerates optic nerve degeneration by alleviating retinal microglia activation. Sci Rep 13 5592 PMID: 37019993

Aakrosh et al (2023) Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer Cells. Cancer Res Commun 3 592-606 PMID: 37077937

Yusuke et al (2021) Osteoblasts derived from mouse mandible enhance tumor growth of prostate cancer more than osteoblasts derived from long bone. J Bone Oncol 26 100346 PMID: 33425674

Evan et al (2022) Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity. Biomolecules 12 PMID: 35454069

Do you know of a great paper that uses MTT from Tocris? Please let us know.

Reviews for MTT

Average Rating: 4.5 (Based on 2 Reviews.)

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Detection of apoptosis via cell proliferation assay MTT was carried out.
By Anonymous on 02/11/2019
Species: Human

Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.

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MTT as a tool to estimate cell proliferation.
By Anonymous on 12/12/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cardiomyocytes

HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.

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