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Description: Ratiometric mass spectrometry mitochondrial hydrogen peroxide (H2O2) probe
Chemical Name: [(3-Boronophenyl)methyl]triphenylphosphonium bromide
Purity: ≥98% (HPLC)
Biological Activity for MitoBoronic acid
MitoBoronic acid (MitoB) is a ratiometric mass spectrometry mitochondrial hydrogen peroxide (H2O2) probe. MitoBoronic acid is taken up into cells and accumulates inside the mitochondrial matrix where it reacts to H2O2 to be converted in MitoP. Measurement of MitoB/MitoP ratio by liquid chromatography tandem mass spectrometry (LC-MS/MS) is an indicator of the mitochondrial matrix H2O2 concentration. This method can be used in vitro and in vivo.
Technical Data for MitoBoronic acid
| M. Wt | 477.14 |
| Formula | C25H23BBrO2P |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1247025-84-8 |
| PubChem ID | 91885451 |
| InChI Key | VDNRROALFUZCBO-UHFFFAOYSA-M |
| Smiles | OB(C1=CC=CC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=C1)O.[Br-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MitoBoronic acid
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 47.71 | 100 | |
| ethanol | 23.86 | 50 with gentle warming |
Preparing Stock Solutions for MitoBoronic acid
The following data is based on the product molecular weight 477.14. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.1 mL | 10.48 mL | 20.96 mL |
| 5 mM | 0.42 mL | 2.1 mL | 4.19 mL |
| 10 mM | 0.21 mL | 1.05 mL | 2.1 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.42 mL |
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Product Datasheets for MitoBoronic acid
References for MitoBoronic acid
References are publications that support the biological activity of the product.
Cochemé et al (2012) Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat.Protoc. 7 946 PMID: 22517261
Gruszczyk et al (2022) Mitochondrial metabolism and bioenergetic function in an anoxic isolated adult mouse cardiomyocyte model of in vivo cardiac ischemia-reperfusion injury. Redox.Biol. 54 102368 PMID: 35749842
Cochemé et al (2011) Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab. 13 340 PMID: 21356523
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Keywords: MitoBoronic acid, MitoBoronic acid supplier, MitoB, MitoP, mass, spectrometry, probe, H2O2, MitoP/MitoB, in, vivo, LC-MS/MS, ratiometric, ROS, Donors, 7949, Tocris Bioscience
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