Minocycline hydrochloride

Pricing Availability   Qty
Description: Antibiotic. Displays neuroprotective effects
Chemical Name: [4S-(4α,4aα,5aα,12aα)]-4,7-Bis(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,10,12,12a,tetrahydroxy-1,11-dioxo-2-naphthacenecarboxamide
Purity: ≥98% (HPLC)
Citations (2)
Reviews (1)
Protocols (1)

Biological Activity for Minocycline hydrochloride

Minocycline hydrochloride is a tetracycline antibiotic. Displays neuroprotective, antiapoptotic, anti-inflammatory and antimicrobial effects. Acts as a matrix metalloproteinase (MMP) inhibitor; attenuates disease severity in mouse models of multiple sclerosis. Orally active and brain penetrant. Allows formation of extended pluripotent stem (EPS) cells in combination with CHIR 99021 (Cat.No. 4423), (S)-(+)-Dimethindene maleate (Cat.No. 1425) and human leukemia inhibitory factor.

For more information about how Minocycline hydrochloride may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Technical Data for Minocycline hydrochloride

M. Wt 493.94
Formula C23H27N3O7.HCl
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 13614-98-7
PubChem ID 54685925
Smiles NC(C4=C(O)[C@@H](N(C)C)[C@@]3([H])[C@](C4=O)(O)C(O)=C2C(C1=C(O)C=CC(N(C)C)=C1C[C@]([H])2C3)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Minocycline hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 49.39 100
water 12.35 25

Preparing Stock Solutions for Minocycline hydrochloride

The following data is based on the product molecular weight 493.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.12 mL 20.25 mL
5 mM 0.4 mL 2.02 mL 4.05 mL
10 mM 0.2 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Minocycline hydrochloride

Certificate of Analysis / Product Datasheet
Select another batch:

References for Minocycline hydrochloride

References are publications that support the biological activity of the product.

Tikka et al (2001) MinCyc, a tetracy. derivative, is neuroprotective against excitotoxicity by inhibiting activation and proliferation of microglia. J.Neurosci. 21 2580 PMID: 11306611

Brundula et al (2002) Targeting leukocyte MMPs and transmigration: MinCyc as a potential therapy for multiple sclerosis. Brain 125 1297 PMID: 12023318

Padi and Kulkarni (2008) MinCyc prevents the development of neuropathic pain, but not acute pain: possible anti-inflammatory and antioxidant mechanisms. Eur.J.Pharmacol. 601 79 PMID: 18952075

Yang et al (2017) Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell. 169 243 PMID: 28388409

If you know of a relevant reference for Minocycline hydrochloride, please let us know.

Keywords: Minocycline hydrochloride, Minocycline hydrochloride supplier, Minocycline, HCl, antibiotics, MMP, matrix, metalloproteinase, inhibitors, inhibits, neuroprotective, Antibiotics, Matrix, Metalloprotease, Stem, Cell, Reprogramming, 3268, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Minocycline hydrochloride (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

2 Citations for Minocycline hydrochloride

Citations are publications that use Tocris products. Selected citations for Minocycline hydrochloride include:

Song et al (2015) MinCyc does not affect long-term potentiation in the anterior cingulate cortex of normal adult mice. Hum Mol Genet 11 25 PMID: 25933605

Yaeger and Trussell (2015) Single granule cells excite Golgi cells and evoke feedback inhibition in the cochlear nucleus. J Neurosci 35 4741 PMID: 25788690

Do you know of a great paper that uses Minocycline hydrochloride from Tocris? Please let us know.

Reviews for Minocycline hydrochloride

Average Rating: 5 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Minocycline hydrochloride?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Blocker of microglia reactivity.
By Luiz Alexandre Magno on 07/16/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain

I have used the antibiotic minocycline to impair activation of microglia in the brain of mice. This drug has anti-inflammatory properties that block microglial reactivity, which can be easily confirmed by expression of the CD68 protein. The drug was diluted in PBS and injected i.p. (75 mg/kg) in mice, twice a day, for 7 days. The image shows that microglial cells (stained for Iba1) do not express CD68 (in red) after treatment with minocycline.

review image

Protocols for Minocycline hydrochloride

The following protocol features additional information for the use of Minocycline hydrochloride (Cat. No. 3268).