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Biological Activity for Laurdan
Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). It is suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS). Laurdan can be used to image lipids in live and fixed cells as well as in whole tissues with multiphoton microscopy. Laurdan has emission maxima of 440 nm and 490 nm in gel and liquid phase membranes, respectively.
Optical Data for Laurdan
Technical Data for Laurdan
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Laurdan
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Laurdan
The following data is based on the product molecular weight 353.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||14.14 mL||70.71 mL||141.42 mL|
|1 mM||2.83 mL||14.14 mL||28.28 mL|
|2 mM||1.41 mL||7.07 mL||14.14 mL|
|10 mM||0.28 mL||1.41 mL||2.83 mL|
References for Laurdan
References are publications that support the biological activity of the product.
Sanchez et al (2012) Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc.Natl.Acad.Sci.U.S.A. 109 7314 PMID: 22529342
Owen et al (2011) Quantitative imaging of membrane lipid order in cells and organisms. Nat.Protoc. 7 24 PMID: 22157973
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Citations for Laurdan
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