Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Fluorescent Lipid Probes and Cell Membrane Stains / Laurdan

Laurdan

Cat. No. 7275 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Polarity sensitive lipid membrane fluorescent probe; used for imaging lipid rafts
Chemical Name: 1-[6-(Dimethylamino)-2-naphthalenyl]-1-dodecanone
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Laurdan

Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). It is suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS). Laurdan can be used to image lipids in live and fixed cells as well as in whole tissues with multiphoton microscopy. Laurdan has emission maxima of 440 nm and 490 nm in gel and liquid phase membranes, respectively.

Optical Data for Laurdan

Laurdan Dye Spectra
λabs 366 nm
λem 497 nm
Extinction Coefficient (ε) 19500 M-1cm-1
Quantum Yield (φ) 0.61
Application Polarity sensitive fluorescent probe for lipid raft and cell membrane investigations
Alternative For C-Laurdan, Di-4-ANEPPDHQ

Technical Data for Laurdan

M. Wt 353.55
Formula C24H35NO
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 74515-25-6
PubChem ID 104983
InChI Key JHDGGIDITFLRJY-UHFFFAOYSA-N
Smiles CCCCCCCCCCCC(C1=CC2=CC=C(N(C)C)C=C2C=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Laurdan

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 7.07 20

Preparing Stock Solutions for Laurdan

The following data is based on the product molecular weight 353.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 14.14 mL 70.71 mL 141.42 mL
1 mM 2.83 mL 14.14 mL 28.28 mL
2 mM 1.41 mL 7.07 mL 14.14 mL
10 mM 0.28 mL 1.41 mL 2.83 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for Laurdan

References are publications that support the biological activity of the product.

Sanchez et al (2012) Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc.Natl.Acad.Sci.U.S.A. 109 7314 PMID: 22529342

Owen et al (2011) Quantitative imaging of membrane lipid order in cells and organisms. Nat.Protoc. 7 24 PMID: 22157973

If you know of a relevant reference for Laurdan, please let us know.

View Related Products by Target

Keywords: Laurdan, Laurdan supplier, fluorescent, probe, imaging, lipid, rafts, membrane, polarity, multiphoton, microscopy, generalized, polarization, GP, and, scanning, fluorescence, correlation, spectroscopy, FCS, Fluorescent, Lipid, Probes, Cell, Membrane, Stains, 7275, Tocris Bioscience

Citations for Laurdan

Citations are publications that use Tocris products.

Currently there are no citations for Laurdan. Do you know of a great paper that uses Laurdan from Tocris? Please let us know.

Reviews for Laurdan

There are currently no reviews for this product. Be the first to review Laurdan and earn rewards!

Have you used Laurdan?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals