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L-Azidonorleucine hydrochloride is an unnatural amino acid; surrogate methionine. Can be incorporated into proteins by a mutant methionyl tRNA synthetase to enable cell-type specific analysis of protein synthesis. For use with bio-orthogonal non-canonical amino-acid tagging (BONCAT) or fluorescent non-canonical amino acid tagging (FUNCAT). Labeling does not affect the normal localization of the labeled proteins.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 208.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||4.79 mL||23.96 mL||47.93 mL|
|5 mM||0.96 mL||4.79 mL||9.59 mL|
|10 mM||0.48 mL||2.4 mL||4.79 mL|
|50 mM||0.1 mL||0.48 mL||0.96 mL|
References are publications that support the biological activity of the product.
Alvarez-Castelao et al (2017) Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat.Biotechnol. 35 1196 PMID: 29106408
Mahdavi et al (2016) Engineered aminoacyl-tRNA synthetase for cell-selective analysis of mammalian protein synthesis. J.Am.Chem.Soc. 138 4278 PMID: 26991063
If you know of a relevant reference for L-Azidonorleucine hydrochloride, please let us know.
Keywords: L-Azidonorleucine hydrochloride, L-Azidonorleucine hydrochloride supplier, unnatural, amino, acid, UAA, ANL, methionine, surrogate, BONCAT, FUNCAT, bio-orthogonal, fluorescent, non-canonical, tagging, methionyl, tRNA, synthetase, metRS, Biochemicals, and, Molecular, Biology, Unnatural, Amino, Acids, 6585, Tocris Bioscience
Citations are publications that use Tocris products.
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