L-655,708

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Cat.No. 1327 - L-655,708 | C18H19N3O4 | CAS No. 130477-52-0
Description: Benzodiazepine inverse agonist; selective for α5-containing GABAA receptors
Chemical Name: 11,12,13,13a-Tetrahydro-7-methoxy-9-oxo-9H-imidazo[1,5-a]pyrrolo[2,1-c][1,4]benzodiazepine-1-carboxylic acid, ethyl ester
Purity: ≥98% (HPLC)
Datasheet
Citations (4)
Literature

Biological Activity

Potent, selective inverse agonist for the benzodiazepine site of GABAA receptors containing the α5 subunit (Ki = 0.45 nM). Displays 50-100-fold selectivity over GABAA receptors containing α1, α2, α3 or α6 subunits in combination with β3 and γ2. Enhances LTP in  a mouse hippocampal slice model and increases spatial learning, without displaying proconvulsant activity.

Compound Libraries

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Technical Data

M. Wt 341.37
Formula C18H19N3O4
Storage Desiccate at +4°C
Purity ≥98% (HPLC)
CAS Number 130477-52-0
PubChem ID 5311203
InChI Key YKYOQIXTECBVBB-AWEZNQCLSA-N
Smiles O=C(N3[C@]([H])2CCC3)C4=C(C=CC(OC)=C4)N1C2=C(C(OCC)=O)N=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 8.53 25

Preparing Stock Solutions

The following data is based on the product molecular weight 341.37. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.93 mL 14.65 mL 29.29 mL
5 mM 0.59 mL 2.93 mL 5.86 mL
10 mM 0.29 mL 1.46 mL 2.93 mL
50 mM 0.06 mL 0.29 mL 0.59 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Atack et al (2006) L-655,708 enhances cognition in rats but is not proconvulsant at a dose selective for α5-containing GABAA receptors. Neuropharmacology 51 1023 PMID: 17046030

Quirk et al (1996) [3H]L-655,708, a novel ligand selective for the benzodiazepine site of GABAA receptors which contain the α5 subunit. Neuropharmacology 35 1331 PMID: 9014149

Sur et al (1998) Rat and human hippocampal α5 subunit-containing γ-aminobutyric acidA receptors have α5α3γ2 pharmacological characteristics. Mol.Pharmacol. 54 928 PMID: 9804628


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Keywords: Selective α5 alpha5 containing a5 GABAA receptors Receptors inverse agonists benzodiazepine L655708 GABAA Receptors

4 Citations for L-655,708

Citations are publications that use Tocris products. Selected citations for L-655,708 include:

Jaiswal et al (2015) Reduction in focal ictal activity following transplantation of MGE interneurons requires expression of the GABAA receptor α4 subunit. J Neurosci 9 127 PMID: 25914623

Seifi et al (2014) Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon. Stem Cells Dev 34 10361 PMID: 25080596

Drexler et al (2013) Enhancing the function of alpha5-subunit-containing GABAA receptors promotes action potential firing of neocortical neurons during up-states. Eur J Pharmacol 703 18 PMID: 23380687

Rüedi-Bettschen et al (2013) Modulation of α5 subunit-containing GABAA receptors alters alcohol drinking by rhesus monkeys. Alcohol Clin Exp Res 37 624 PMID: 23126673


Do you know of a great paper that uses L-655,708 from Tocris? If so please let us know.

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