Janelia Fluor® 646, Azide

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Cat.No. 7088 - Janelia Fluor 646, Azide | C37H44N6O6Si
Description: Red dye (Reactive group: azide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, and live cell imaging
Alternative Names: JF 646
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)carbamoyl)benzoate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Janelia Fluor® 646, Azide

Janelia Fluor® 646, Azide is a red fluorogenic fluorescent dye, supplied as an azide for click chemistry. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54; Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 646, Azide

Janelia Fluor<sup>®</sup> 646, Azide Dye Spectra
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group Azide
Reactivity Click
Cell Permeable Yes
Correction Factor 280 0.19
Application dSTORM, STED, confocal
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Janelia Fluor® 646, Azide

M. Wt 696.88
Formula C37H44N6O6Si
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 646, Azide

Solvent Max Conc. mg/mL Max Conc. mM
DMF 34.84 50

Preparing Stock Solutions for Janelia Fluor® 646, Azide

The following data is based on the product molecular weight 696.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.87 mL 14.35 mL 28.7 mL
2.5 mM 0.57 mL 2.87 mL 5.74 mL
5 mM 0.29 mL 1.43 mL 2.87 mL
25 mM 0.06 mL 0.29 mL 0.57 mL

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Product Datasheets for Janelia Fluor® 646, Azide

References for Janelia Fluor® 646, Azide

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 12 244 PMID: 25599551

If you know of a relevant reference for Janelia Fluor® 646, Azide, please let us know.

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Citations for Janelia Fluor® 646, Azide

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