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Submit ReviewJanelia Fluor® 570, NHS ester New
Description: Orange dye (Reactive group: NHS ester). Application: Suitable for live cell imaging
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-thioxanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate trifluoroacetate
Purity: ≥85% (HPLC)
Biological Activity for Janelia Fluor® 570, NHS ester
Key information: Janelia Fluor® 570, NHS ester is an orange photosensitizer for proximity labeling experiments; supplied with an NHS ester reactive for the labeling of primary amines.
Suitable for live cell applications. Upon absorption of light energy, photosensitizers in the triplet excited state interact with molecular oxygen resulting in the formation of singlet oxygen and other ROS that can be leveraged to manipulate or interrogate biological systems.
Used for: proximity labeling experiments.
Application: photosensitizer-dependent oxidation and capture by amine (POCA) platforms reporting intracellular interactomes for both proteins and lipids such as cholesterol. Also suitable for chromophore-assisted light inactivation (CALI) of proteins in live cells; targeted ablation of cells; DAB photopolymerization for contrast in light and electron microscopy (EM).
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. Haloalkane and SNAP-tag®. Excitation and emission maxima (λ) are 570 nm and 593 nm, respectively; quantum yield φ = 0.63; extinction coefficient ε = 83,600 M-1cm-1; singlet oxygen quantum yield φSO = 0.088; lactone-zwitterion equilibrium constant (KL-Z) = 2.24. Janelia Fluor® 570, NHS ester is net neutral and cell permeable.
Protocol: for cell-based POCA: cells are dosed with JF570-NHS (100 nM, 10 min) followed by washout to remove excess reagent (2 x 20 min), addition of PA (10 mM) and irradiation (170,000 Lux, 5 min,15 W yellow LED). After cell lysis, click chemistry and SDS-PAGE are performed.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer custom conjugation antibody services.
SNAP-tag® is a registered trademark of New England BioLabs, Inc.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 570, NHS ester
| Emission Color | Orange |
|---|---|
| λabs | 570 nm |
| λem | 593 nm |
| Extinction Coefficient (ε) | 83,600 M-1cm-1 |
| Quantum Yield (φ) | 0.63 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | POCA |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Janelia Fluor® 570, NHS ester
| M. Wt | 681.64 |
| Formula | C31H25N3O6S.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥85% (HPLC) |
| InChI Key | ITQBSGNYLQKYTI-UHFFFAOYSA-N |
| Smiles | OC(C(F)(F)F)=O.O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3S5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Janelia Fluor® 570, NHS ester
References for Janelia Fluor® 570, NHS ester
References are publications that support the biological activity of the product.
Binns et al (2020) Rational design of bioavailable photosensitizers for manipulation and imaging of biological systems. Cell.Chem.Bio. 27 1063 PMID: 32698018
Becker et al (2026) Photosensitizer proximity labeling captures the lipid and protein interactomes. Nat.Chem.Biol. PMID: 41652064
If you know of a relevant reference for Janelia Fluor® 570, NHS ester, please let us know.
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Protocols for Janelia Fluor® 570, NHS ester
The following protocol features additional information for the use of Janelia Fluor® 570, NHS ester (Cat. No. 8983).
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents