Janelia Fluor® 635, Maleimide

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Description: Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging
Chemical Name: 4-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Datasheet
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Literature (1)

Biological Activity for Janelia Fluor® 635, Maleimide

Key information: Janelia Fluor® 635, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 664 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company R&D Systems.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 635, Maleimide

Janelia Fluor<sup>®</sup> 635, Maleimide Dye Spectra
λabs 645 nm
λem 664 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Janelia Fluor® 635, Maleimide

M. Wt 654.75
Formula C35H32F2N4O5Si
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key MDMNIGSKRXKBRM-UHFFFAOYSA-N
Smiles O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 635, Maleimide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.55 10

Preparing Stock Solutions for Janelia Fluor® 635, Maleimide

The following data is based on the product molecular weight 654.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 15.27 mL 76.37 mL 152.73 mL
0.5 mM 3.05 mL 15.27 mL 30.55 mL
1 mM 1.53 mL 7.64 mL 15.27 mL
5 mM 0.31 mL 1.53 mL 3.05 mL

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Product Datasheets for Janelia Fluor® 635, Maleimide

References for Janelia Fluor® 635, Maleimide

References are publications that support the biological activity of the product.

If you know of a relevant reference for Janelia Fluor® 635, Maleimide, please let us know.

Keywords: Janelia Fluor® 635, Maleimide, Janelia Fluor® 635, Maleimide supplier, JF635, Janelia, Fluor, 635-Maleimide, fluorescent, dyes, microscopy, cell, permeable, super, resolution, flow, cytometry, Sulfhydryl, Reactive, Dyes, Flow, Cytometry, 8027, Tocris Bioscience

Citations for Janelia Fluor® 635, Maleimide

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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