Janelia Fluor® 635, Maleimide

Pricing Availability   Qty
Description: Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging
Chemical Name: 4-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Janelia Fluor® 635, Maleimide

Key information: Janelia Fluor® 635, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 664 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company R&D Systems.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 635, Maleimide

Janelia Fluor<sup>®</sup> 635, Maleimide Dye Spectra
λabs 645 nm
λem 664 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Janelia Fluor® 635, Maleimide

M. Wt 654.75
Formula C35H32F2N4O5Si
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 635, Maleimide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 6.55 10

Preparing Stock Solutions for Janelia Fluor® 635, Maleimide

The following data is based on the product molecular weight 654.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 15.27 mL 76.37 mL 152.73 mL
0.5 mM 3.05 mL 15.27 mL 30.55 mL
1 mM 1.53 mL 7.64 mL 15.27 mL
5 mM 0.31 mL 1.53 mL 3.05 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Janelia Fluor® 635, Maleimide

References for Janelia Fluor® 635, Maleimide

References are publications that support the biological activity of the product.

If you know of a relevant reference for Janelia Fluor® 635, Maleimide, please let us know.

Keywords: Janelia Fluor® 635, Maleimide, Janelia Fluor® 635, Maleimide supplier, JF635, Janelia, Fluor, 635-Maleimide, fluorescent, dyes, microscopy, cell, permeable, super, resolution, flow, cytometry, Sulfhydryl, Reactive, Dyes, Flow, Cytometry, 8027, Tocris Bioscience

Citations for Janelia Fluor® 635, Maleimide

Citations are publications that use Tocris products.

Currently there are no citations for Janelia Fluor® 635, Maleimide. Do you know of a great paper that uses Janelia Fluor® 635, Maleimide from Tocris? Please let us know.

Reviews for Janelia Fluor® 635, Maleimide

There are currently no reviews for this product. Be the first to review Janelia Fluor® 635, Maleimide and earn rewards!

Have you used Janelia Fluor® 635, Maleimide?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals