Janelia Fluor® 549, Maleimide

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Cat.No. 6500 - Janelia Fluor 549, Maleimide | C33H28N4O6.C2HF3O2
Description: Yellow dye (Reactive group: maleimide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity for Janelia Fluor® 549, Maleimide

Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 549, Maleimide

Janelia Fluor<sup>®</sup> 549, Maleimide Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for Janelia Fluor® 549, Maleimide

M. Wt 690.63
Formula C33H28N4O6.C2HF3O2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2.[CF3]C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 549, Maleimide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 69.06 100

Preparing Stock Solutions for Janelia Fluor® 549, Maleimide

The following data is based on the product molecular weight 690.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.45 mL 7.24 mL 14.48 mL
5 mM 0.29 mL 1.45 mL 2.9 mL
10 mM 0.14 mL 0.72 mL 1.45 mL
50 mM 0.03 mL 0.14 mL 0.29 mL

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Product Datasheets for Janelia Fluor® 549, Maleimide

Certificate of Analysis / Product Datasheet
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References for Janelia Fluor® 549, Maleimide

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy Nat.Methods. 12 244 PMID: 25599551


If you know of a relevant reference for Janelia Fluor® 549, Maleimide, please let us know.

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Citations for Janelia Fluor® 549, Maleimide

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Protocols for Janelia Fluor® 549, Maleimide

The following protocol features additional information for the use of Janelia Fluor® 549, Maleimide (Cat. No. 6500).

Literature in this Area

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