Janelia Fluor® 549, Maleimide

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Description: Yellow dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate
Purity: ≥95% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for Janelia Fluor® 549, Maleimide

Key information: Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 549, Maleimide

Janelia Fluor<sup>®</sup> 549, Maleimide Dye Spectra
Emission Color Yellow
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal Imaging, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Janelia Fluor® 549, Maleimide

M. Wt 690.63
Formula C33H28N4O6.C2HF3O2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2.[CF3]C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 549, Maleimide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 69.06 100

Preparing Stock Solutions for Janelia Fluor® 549, Maleimide

The following data is based on the product molecular weight 690.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.45 mL 7.24 mL 14.48 mL
5 mM 0.29 mL 1.45 mL 2.9 mL
10 mM 0.14 mL 0.72 mL 1.45 mL
50 mM 0.03 mL 0.14 mL 0.29 mL

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Product Datasheets for Janelia Fluor® 549, Maleimide

Certificate of Analysis / Product Datasheet
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References for Janelia Fluor® 549, Maleimide

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy Nat.Methods. 12 244 PMID: 25599551

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor® 549, Maleimide, please let us know.

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Citations for Janelia Fluor® 549, Maleimide

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Protocols for Janelia Fluor® 549, Maleimide

The following protocol features additional information for the use of Janelia Fluor® 549, Maleimide (Cat. No. 6500).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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