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Janelia Fluor® 549, Maleimide
Biological Activity for Janelia Fluor® 549, Maleimide
Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Maleimide
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Correction Factor 280||0.169|
|Application||Confocal Imaging, dSTORM, STED, Flow Cytometry|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for Janelia Fluor® 549, Maleimide
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 549, Maleimide
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Janelia Fluor® 549, Maleimide
The following data is based on the product molecular weight 690.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.45 mL||7.24 mL||14.48 mL|
|5 mM||0.29 mL||1.45 mL||2.9 mL|
|10 mM||0.14 mL||0.72 mL||1.45 mL|
|50 mM||0.03 mL||0.14 mL||0.29 mL|
Product Datasheets for Janelia Fluor® 549, Maleimide
References for Janelia Fluor® 549, Maleimide
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy Nat.Methods. 12 244 PMID: 25599551
If you know of a relevant reference for Janelia Fluor® 549, Maleimide, please let us know.
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Citations for Janelia Fluor® 549, Maleimide
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Protocols for Janelia Fluor® 549, Maleimide
The following protocol features additional information for the use of Janelia Fluor® 549, Maleimide (Cat. No. 6500).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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