Indo 1AM

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Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for Indo 1AM

Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry..

This product is typically prepared in DMSO.

Optical Data for Indo 1AM

Emission Color Blue
λabs 355 nm
λem 474 nm
Closest Laser line 350 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescence Microscopy

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Technical Data for Indo 1AM

M. Wt 1009.91
Formula C47H51N3O22
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 112926-02-0
PubChem ID 123918

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for Indo 1AM

References are publications that support the biological activity of the product.

Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314

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Keywords: Indo 1AM, Indo 1AM supplier, Indo1AM, fluorescent, calcium, Ca2+, indicator, flow, cytometry, cell, permeable, Ion, Indicators, Fluorescent, 6704, Tocris Bioscience

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