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Biological Activity for Indo 1AM
Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry.
Technical Data for Indo 1AM
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Indo 1AM
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Indo 1AM
The following data is based on the product molecular weight 1009.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.01 mM||99.02 mL||495.09 mL||990.19 mL|
|0.05 mM||19.8 mL||99.02 mL||198.04 mL|
|0.1 mM||9.9 mL||49.51 mL||99.02 mL|
|0.5 mM||1.98 mL||9.9 mL||19.8 mL|
References for Indo 1AM
References are publications that support the biological activity of the product.
Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314
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