Indo 1AM

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Cat.No. 6704 - Indo 1AM | C47H51N3O22 | CAS No. 112926-02-0
Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry.

Technical Data

M. Wt 1009.91
Formula C47H51N3O22
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 112926-02-0
PubChem ID 123918
InChI Key CAWBRCOBJNWRLK-UHFFFAOYSA-N
Smiles CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.01 1

Preparing Stock Solutions

The following data is based on the product molecular weight 1009.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 99.02 mL 495.09 mL 990.19 mL
0.05 mM 19.8 mL 99.02 mL 198.04 mL
0.1 mM 9.9 mL 49.51 mL 99.02 mL
0.5 mM 1.98 mL 9.9 mL 19.8 mL

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References

References are publications that support the biological activity of the product.

Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314


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Keywords: Indo 1AM, Indo 1AM supplier, Indo1AM, fluorescent, calcium, Ca2+, indicator, flow, cytometry, cell, permeable, Ion, Indicators, 6704, Tocris Bioscience

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