IDE 1

Pricing Availability Delivery Time Qty
Cat.No. 4015 - IDE 1 | C15H18N2O5 | CAS No. 1160927-48-9
Description: Induces definitive endoderm formation in mouse and human ESCs
Chemical Name: 1-[2-[(2-Carboxyphenyl)methylene]hydrazide]heptanoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Literature

Biological Activity

Induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.

Licensing Information

Sold for research purpose under license from Harvard University.

Compound Libraries

IDE 1 is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 306.31
Formula C15H18N2O5
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1160927-48-9
PubChem ID 66974738
InChI Key ABKJCDILEUEJSH-UHFFFAOYSA-N
Smiles OC(=O)CCCCCC(=O)NN=CC1=CC=CC=C1C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.63 100
ethanol 7.66 25
2eq. NaOH 30.63 100

Preparing Stock Solutions

The following data is based on the product molecular weight 306.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.32 mL 32.65 mL
5 mM 0.65 mL 3.26 mL 6.53 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Borowiak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624

Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285

Filby et al (2011) Stimulation of activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501


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Keywords: IDE1 stem cells induces definitive endoderm human mouse ESCs tgfb transforming growth factors beta b β signaling signalling nodal ESCs and iPSC

2 Citations for IDE 1

Citations are publications that use Tocris products. Selected citations for IDE 1 include:

Xu et al (2015) Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol. Stem Cell Res 6 193 PMID: 26438426

Kearns et al (2013) Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. J Cell Mol Med 11 1003 PMID: 23917481


Do you know of a great paper that uses IDE 1 from Tocris? If so please let us know.

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Literature in this Area

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Cancer Research Product Guide

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Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for IDE 1

Protocols

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