Induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.
Sold for research purpose under license from Harvard University.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 306.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.26 mL||16.32 mL||32.65 mL|
|5 mM||0.65 mL||3.26 mL||6.53 mL|
|10 mM||0.33 mL||1.63 mL||3.26 mL|
|50 mM||0.07 mL||0.33 mL||0.65 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Borowiak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624
Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285
Filby et al (2011) Stimulation of activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501
If you know of a relevant reference for IDE 1, please let us know.
Keywords: IDE1 stem cells induces definitive endoderm human mouse ESCs tgfb transforming growth factors beta b β signaling signalling nodal ESCs and iPSC
2 Citations for IDE 1
Citations are publications that use Tocris products. Selected citations for IDE 1 include:
Xu et al (2015) Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol. Stem Cell Res 6 193 PMID: 26438426
Kearns et al (2013) Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. J Cell Mol Med 11 1003 PMID: 23917481
Do you know of a great paper that uses IDE 1 from Tocris? If so please let us know.
Literature in this Area
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.