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Induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.
This compound is mixture of E/Z isomers.
Sold for research purpose under license from Harvard University.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 306.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.26 mL||16.32 mL||32.65 mL|
|5 mM||0.65 mL||3.26 mL||6.53 mL|
|10 mM||0.33 mL||1.63 mL||3.26 mL|
|50 mM||0.07 mL||0.33 mL||0.65 mL|
References are publications that support the biological activity of the product.
Borowiak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624
Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285
Filby et al (2011) Stimulation of AVNA/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501
If you know of a relevant reference for IDE 1, please let us know.
Keywords: IDE 1, IDE 1 supplier, IDE1, stem, cells, induces, definitive, endoderm, human, mouse, ESCs, tgfb, transforming, growth, factors, beta, b, β, signaling, signalling, nodal, and, iPSC, 4015, Tocris Bioscience
2 Citations for IDE 1
Citations are publications that use Tocris products. Selected citations for IDE 1 include:
Kearns et al (2013) Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. J Cell Mol Med 11 1003 PMID: 23917481
Xu et al (2015) Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol. Stem Cell Res 6 193 PMID: 26438426
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.