IDE 1

Pricing Availability Delivery Time Qty
Cat.No. 4015 - IDE 1 | C15H18N2O5 | CAS No. 1160927-48-9
Description: Induces definitive endoderm formation in mouse and human ESCs
Chemical Name: 1-[2-[(2-Carboxyphenyl)methylene]hydrazide]heptanoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews
Literature

Biological Activity

Induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.

This compound is mixture of E/Z isomers.

Licensing Information

Sold for research purpose under license from Harvard University.

Compound Libraries

IDE 1 is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 306.31
Formula C15H18N2O5
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1160927-48-9
PubChem ID 66974738
InChI Key ABKJCDILEUEJSH-UHFFFAOYSA-N
Smiles OC(=O)CCCCCC(=O)NN=CC1=CC=CC=C1C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.63 100
2eq. NaOH 30.63 100

Preparing Stock Solutions

The following data is based on the product molecular weight 306.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.32 mL 32.65 mL
5 mM 0.65 mL 3.26 mL 6.53 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the biological activity of the product.

Borowiak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624

Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285

Filby et al (2011) Stimulation of activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501


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Keywords: IDE 1, IDE 1 supplier, IDE1, stem, cells, induces, definitive, endoderm, human, mouse, ESCs, tgfb, transforming, growth, factors, beta, b, β, signaling, signalling, nodal, and, iPSC, 4015, Tocris Bioscience

2 Citations for IDE 1

Citations are publications that use Tocris products. Selected citations for IDE 1 include:

Kearns et al (2013) Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. J Cell Mol Med 11 1003 PMID: 23917481

Xu et al (2015) Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol. Stem Cell Res 6 193 PMID: 26438426


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